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Volume 142, Issue 2

Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme Free

Abstract

Summary: A novel methylotroph, strain M2, capable of utilizing methanesulfonic acid (MSA) as a sole source of carbon and energy was the subject of these investigations. The initial step in the biodegradative pathway of MSA in strain M2 involved an inducible NADH-specific monooxygenase enzyme (MSAMO). Partial purification of MSAMO from cell-free extracts by ion-exchange chromatography led to the loss of MSAMO activity. Activity was restored by the mixing of three distinct protein fractions designated A, B and C. The reconstituted enzyme had a narrow substrate specificity relative to crude cell-free extracts. Addition of FAD and ferrous ions to the reconstituted enzyme complex resulted in a fivefold increase in enzyme activity, suggesting the loss of FAD and ferrous ion from the multicomponent enzyme on purification. Analysis of mutants of strain M2 defective in the metabolism of C compounds indicated that methanol was not an intermediate in the degradative pathway of MSA and also confirmed the involvement of a multicomponent enzyme in the degradation of MSA by methylotroph strain M2.

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Keyword(s): alkyl sulfonates , C1metabolism , methanesulfonic acid (MSA) , methylotrophy and multicomponent enzyme
© Society for General Microbiology 1996
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1996-02-01
2024-04-16
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Metabolism of methanesulfonic acid involves a multicomponent monooxygenase enzyme
Microbiology 142, 251 (1996); https://doi.org/10.1099/13500872-142-2-251
/content/journal/micro/10.1099/13500872-142-2-251
/content/journal/micro/10.1099/13500872-142-2-251
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