RT Journal Article SR Electronic(1) A1 Togni, Giuseppe A1 Sanglard, Dominique A1 Quadroni, Manfredo A1 Foundling, Stephen I. A1 Monod, MichelYR 1996 T1 Acid Proteinase Secreted by Candida Tropicalis: Functional Analysis of Preproregion Cleavages in C. Tropicalis and Saccharomyces Cerevisiae JF Microbiology, VO 142 IS 3 SP 493 OP 503 DO https://doi.org/10.1099/13500872-142-3-493 PB Microbiology Society, SN 1465-2080, AB The 40 kDa secreted aspartyl proteinase (Sapt1) of Candida tropicalis is a pepsin-like enzyme encoded by the SAPT1 gene. According to the deduced amino acid sequence, Sapt1 has a putative preproregion of 60 amino acids preceding the mature enzyme. Maturation and processing of Sapt1 was analysed in C. tropicalis and Saccharomyces cerevisiae strains expressing wild-type or mutated forms of SAPT1. In S. cerevisiae the glycosylated 46 kDa proenzyme was converted to the mature 40 kDa form of Sapt1 by KEX2-dependent proteolytic cleavage following the Lys59-Arg60 sequence. The replacement of Lys59-Arg60 by Lys59-Gly60 revealed that the precursor can be processed by an autocatalytic cleavage. This alternative processing pathway to produce mature Sapt1 is less efficient than the Kex2-mediated pathway. Finally, it was shown that in C. tropicalis and S. cerevisiae the removal of the proregion was a prerequisite for the secretion of Sapt1., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-142-3-493