1887

Abstract

A homologue of the ‘ferric uptake regulation’ gene () was isolated from the cyanobacterium sp. strain PCC 7942 by an -based repression assay’. The assay uses a reporter-gene construct containing the promoter region of the iron-regulated cyanobacterial gene , fused to the coding region for chloramphenicol acetyltransferase. The isolated gene codes for a protein that has 41 % sequence similarity (36% identity) to Fur from and contains the putative iron-binding motif found in the Fur proteins of purple bacteria. No significant similarity was found to the DxtR repressor that regulates the expression of toxin and siderophore production in Gram-positive bacteria. Insertional mutagenesis of the cloned cyanobacterial gene led to the creation of heteroallelic mutants that showed iron-deficiency symptoms in iron-replete medium, including the constitutive production of flavodoxin and of hydroxamate siderophores. Failure to eliminate wild-type copies of the gene from the polyploid genome of 7942 implies that in this cyanobacterium Fur may have essential functions in addition to the regulation of genes involved in iron scavenging or photosynthetic electron transport.

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1996-06-01
2024-03-29
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References

  1. Allen M.M. Simple conditions for growth of unicellular blue-green algae on plates. J Phycol 1968; 4:1–3
    [Google Scholar]
  2. De Baar H.J.W., De Jong J.T.M., Bakker D.C.E., Löscher B.M., Veth C., Bathmann U., Smetacek V. Importance of iron for plankton blooms and carbon dioxide drawdown in the Southern Ocean. Nature 1995; 373:412–415
    [Google Scholar]
  3. Berish S.A., Subbarao S., Chen C.Y., Trees D.L., Morse S.A. Identification and cloning of a fur homologue from Neisseria gonorrhoeae. Infect Immun 1993; 61:4599–4606
    [Google Scholar]
  4. Binder B.J., Chisholm S.W. Relationship between DNA cycle and growth rate in Synechococcus sp. strain PCC 6301. J Bacterial 1990; 172:2313–2319
    [Google Scholar]
  5. Braun V., Hantke K. Genetics of bacterial iron transport. In CRC Handbook of Molecular Microbial Iron Chelates 1991 Edited by Winkelmann G. Boca Raton, FL: CRC Press; pp 107–138
    [Google Scholar]
  6. Brown C.M., Trick C.G. Response of the cyanobacterium, Oscillatoria tenuis, to low iron environments: the effect on the growth rate and evidence for siderophore production. Arch Microbiol 1992; 157:349–354
    [Google Scholar]
  7. Burnap R.L., Troyan T., Sherman L.A. The highly abundant chlorophyll-protein complex of iron-deficient Synechococcus sp PCC 7942 (CP43') is encoded by the isiA gene. Plant Physiol 1993; 103:893–902
    [Google Scholar]
  8. Chang A.C.Y.C., Cohen S.N. Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacterial 1978; 134:1141–1156
    [Google Scholar]
  9. Coleman J.R., Grossman A.R. Biosynthesis of carbonic anhydrase in Chlamydomonas reinhardtii during adaptation to low C02. Proc Natl Acad Sei USA 1984; 81:6049–6053
    [Google Scholar]
  10. Coy M., Neilands J.B. Structural dynamics and functional domains of the Fur protein. Biochemistry 1991; 30:8201–8210
    [Google Scholar]
  11. Csäky T.Z. On the estimation of bound hydroxylamine in biological materials. Acta Chem Scand 1948; 2:450–454
    [Google Scholar]
  12. Ferreira F., Straus N.A. Iron deprivation in cyanobacteria. J Appl Phycol 1994; 6:199–210
    [Google Scholar]
  13. Golden S.S. The mutagenesis of cyanobacteria by classical and gene-transfer-based methods. Methods Enzymol 1988; 167:714–727
    [Google Scholar]
  14. Hantke K. Regulation of ferric iron transport in Escherichia coli K12: isolation of a constitutive mutant. Mol Gen Genet 1981; 182:288–292
    [Google Scholar]
  15. Hantke K. Selection procedure for deregulated iron transport mutants (fur) in Escherichia coli K12: fur not only affects iron metabolism. Mol Gen Genet 1987; 210:135–139
    [Google Scholar]
  16. Hennecke H. Regulation of bacterial gene expression by metal-protein complexes. Mol Microbiol 1990; 4:1621–1628
    [Google Scholar]
  17. Karkhoff-Schweizer R.R., Schryvers A.B., Schweizer H.P. Cloning and sequence analysis of the fur gene encoding an iron-regulatory protein of Neisseria meningitidis. Gene 1994; 141:139–140
    [Google Scholar]
  18. Kump L. Bacteria forge a new link. Nature 1993; 362:790–791
    [Google Scholar]
  19. Lau R.H., Straus N.A. Versatile shuttle cloning vectors for the unicellular cyanobacterium Anacystis nidulans R2. FEMS Microbiol Eett 1985; 27:253–256
    [Google Scholar]
  20. Laudenbach D.E., Straus N.A. Characterization of a cyanobacterial iron stress-induced gene similar to psbC. J Bacterial 1988; 170:5018–5026
    [Google Scholar]
  21. Martin J.H., Fitzwater S.E. Iron deficiency limits phytoplankton growth in the north-east Pacific subarctic. Nature 1988; 331:341–343
    [Google Scholar]
  22. Martin J.H., Coale K.H., Johnson K.S., Fitzwater S.E., Gordon R.M., Tanner S.J., Hunter C.N., Elrod V.A., Nowicki J.L., Coley T.L., Barber R.T., Lindley S., Watson A.J., Van Scoy K., Law C.S., Liddicoat M.I., Ling R., Stanton T., Stocjel J., Collins C., Anderson A., Bidigare R., Ondrusek M., Latasa M., Millero F.J., Lee K., Yao W., Zhang J.Z., Friederich G., Sakamoto C., Chavez F., Buck K., Kolber Z., Greene R., Falkowski P., Chisholm S., W., Hoge F., Swift R., Yungel J., Turner S., Nightingale P., Hatton A., Liss P., Tindale N.W. Testing the iron hypothesis in ecosystems of the equatorial Pacific Ocean. Nature 1994; 371:123–129
    [Google Scholar]
  23. Miller J.H. A Short Course in Bacterial Genetics 1992 Cold Spring Harbor, NY: Cold Spring Harbor Laboratory; pp 71–75
    [Google Scholar]
  24. Prince R.W., Cox C.D., Vasil M.L. Coordinate regulation of siderophore and exotoxin A production: molecular cloning and sequencing of the Pseudomonas aeruginosa fur gene. J Bacteriol 1993; 175:2589–2598
    [Google Scholar]
  25. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: a 'Laboratory Manual 1989 2nd edn Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;1860–1875
    [Google Scholar]
  26. Sandmann G., Malkin R. Iron-sulfur centers and activities of the photosynthetic electron transport chain in iron-deficient cultures of the blue-green alga Aphanocapsa. Plant Physiol 1983; 73:724–728
    [Google Scholar]
  27. Straus N.A. Iron deprivation: physiology and gene regulation. In The Molecular Biology of Cyanobacteria 1994 Edited by Bryant D.A. Boston: Dordrecht: Kluwer; pp 731–735
    [Google Scholar]
  28. Tao T., Murphy J.R. Binding of the metalloregulatory protein DtxR to the diphtheria tox operator requires a divalent heavy metal ion and protects the palindromic sequences from DNase I digestion. J Biol Chem 1992; 267:21761–21764
    [Google Scholar]
  29. Tolmasky M.E., Wertheimer A.M., Actis L.A., Crosa J.H. Characterization of the Vibrio anguillarum fur gene: role in regulation of expression of the FatA outer membrane protein and catechols. J Bacteriol 1994; 176:213–220
    [Google Scholar]
  30. Trick C., G. & Kerry A. Isolation and purification of siderophores produced by cyanobacteria, Synechococcus sp PCC 7942 and Anahaena ATCC 29413. Curr Microbiol 1992; 24:241–245
    [Google Scholar]
  31. Wang Z., Schmitt M.P., Holmes R.K. Characterization of mutations that inactivate the diphtheria toxin repressor gene (dtxR). Infect Immun 1994; 62:1600–1608
    [Google Scholar]
  32. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors. Gene 1985; 33:103–199
    [Google Scholar]
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