1887

Abstract

The fungus-feeding beetle, , consumed equal quantities of young mycelia, fewer phialides bearing mature spores and much fewer phialides bearing developing spores of compared to those of when tested in diet choice assays. The degree to which specific fungal structures were consumed was inversely related to the localization of high levels of restrictocin, a ribosome-inactivating protein, to those structures. Pure restrictocin added to the insect diet at 1000 p.p.m. killed 38.5% of larvae and 62.5% of larvae in 48 h, but did not affect adults or larvae over the same interval. In diet choice assays, 1000 p.p.m. of restrictocin deterred feeding by adult and compared to control diets. Thus, restrictocin production and localization may have a natural defensive role against insect feeding at times critical to spore formation by , and may have potential as an insect control agent.

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1996-06-01
2024-04-19
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References

  1. Agrawal N.S., Christensen C.M., Hodson A.C. Grain storage fungi associated with the granary weevil. J Econ Entomol 1957; 50:659–663
    [Google Scholar]
  2. Armitage D.M., George C.L. The effect of three species of mites upon fungal growth on wheat. Exp Appl Acarol 1986; 2:111–124
    [Google Scholar]
  3. Bass H.W., Webster C., O'Brian G.R., Roberts J.K.M. A maize ribosome-inactivating protein is controlled by the transcriptional activator Opaque-2. Plant Cell 1992; 4:225–234
    [Google Scholar]
  4. Brandhorst T., Kenealy W.R. Production and localization of restrictocin in Aspergillus restrictus. J Gen Microbiol 1992; 138:1429–1435
    [Google Scholar]
  5. Brandhorst T., Yang R., Kenealy W.R. Heterologous expression of the cytotoxin restrictocin in Aspergillus nidulans and Aspergillus niger. Protein Expr Pur if 1994; 5:486–497
    [Google Scholar]
  6. Conde F.P., Fernandez-Puentes C., Montero M.T.V., Vazquez D. Protein toxins that catalytically inactivate ribosomes from eukaryotic microorganisms Studies on the mode of action of alpha sarcin, mitogillin and restrictocin: response to alpha-sarcin antibodies. FEMS Microbiol Lett 1978; 4:349–355
    [Google Scholar]
  7. Cullen D., Gray G., Wilson L., Hayenga K., Lamsa M., Rey M., Norton S., Berka R. Controlled expression and secretion of bovine chymosin in Aspergillus nidulans. Bio technology 1987a; 5:369–376
    [Google Scholar]
  8. Cullen D., Wilson L.J., Grey G.L., Henner D.J., Turner G., Ballance D.J. Sequence and centromere proximal location of a transformation enhancing fragment ansi from Aspergillus nidulans. Nucleic Acids Res 1987b; 15:9163–9175
    [Google Scholar]
  9. Dowd P.F. Toxicological and biochemical interactions of the fungal metabolites fusaric acid and kojic acid with xenobiotics in Heliothis %ea (F and Spodoptera frugiperda (J. E. Smith). Pestic Biochem Physiol 1988; 32:123–134
    [Google Scholar]
  10. Dowd P.F. Responses of Carpophilus hemipterus larvae and adults to selected secondary metabolites of maize. Entomol Exp Appl 1990; 54:29–36
    [Google Scholar]
  11. Dowd P.F. Insect interactions with mycotoxin-producing fungi and their hosts. In Handbook of Applied Mycology Mycotoxins in Ecological Systems 1992a Edited by Bhatnagar D., Lillehoj E.B., Arora D.K. New York: Marcel Dekker; 5 pp 137–155
    [Google Scholar]
  12. Dowd P.F. Detoxification of mycotoxins by insects. In Molecular Mechanisms of Insecticide Resistance: Diversity Among Insects 1992b Edited by Mullin C.A., Scott J.G. Washington, DC: American Chemical Society; pp 264–275
    [Google Scholar]
  13. Dowd P.F. Enhanced maize (Zea mays L. pericarp browning: associations with insect resistance and involvement of oxidizing enzymes. J Chem Ecol 1994; 20:2777–2803
    [Google Scholar]
  14. Dowd P.F. Sap beetles and mycotoxins in maize. Food Addit Contam 1995; 12:497–508
    [Google Scholar]
  15. Dowd P.F., Weber G.M. A labor-saving method for rearing a corn sap beetle, Carpophilus freemani Dobson (Coleoptera: Nitidulidae) on pinto bean-based diet. J Agric Entomol 1991; 8:149–153
    [Google Scholar]
  16. Frey D., Oldfield R.J., Bridger R.G. Color Atlas of Pathogenic Fungi 1979 Chicago: Yearbook Medical Publishers;
    [Google Scholar]
  17. Gatehouse A.M.R., Barbieri L., Stirpe F., Croy R.R.D. Effects of ribosome inactivating proteins on insect development -differences between Lepidoptera and Coleoptera. Entomol Exp Appl 1990; 54:43–51
    [Google Scholar]
  18. Gatehouse A.M.R., Shi Y., Powell K.S., Brough G., Hilder V.A., Hamilton W.D.O., Newell C.A., Merryweather A., Boulter D., Gatehouse J.A. Approaches to insect resistance using transgenic plants. Philos Trans R Soc Lond B 1993; 342:279–286
    [Google Scholar]
  19. Gould F., Anderson A., Landis D., Van Mellaert H. Feeding behavior and growth of Heliothis virescens larvae on diets containing Bacillus thuringiensis formulations or endotoxins. Entomol Exp Appl 1991; 58:199–210
    [Google Scholar]
  20. Griffiths D.A., Hodson A.C., Christensen C.M. Grain storage fungi associated with mites. J Econ Entomol 1959; 52:514–518
    [Google Scholar]
  21. Gupta S.C., Asnani V.L., Khare B.P. Effect of the opaque-2 gene in maize (Zea mays L. on the extent of infestation by Sitophilus oryzae L. J Stored Prod Res 1970; 6:191–194
    [Google Scholar]
  22. Jewell F.F. Insect transmission of oak wilt. Phytopathology 1956; 46:244–257
    [Google Scholar]
  23. Jimenez A., Vazquez D. Plant and fungal protein and glycoprotein toxins inhibiting eukaryote protein synthesis. Amu Rev Microbiol 1985; 39:649–672
    [Google Scholar]
  24. Moller W., J. & Devay J.E. Insect transmission of Ceratocysus fimbriata in deciduous fruit orchards. Phytopathology 1968; 58:1499–1508
    [Google Scholar]
  25. Rajendran N., Sivamani E., Jayaraman K. Expression of the insecticidal crystal protein gene from a Gram-positive Bacillus thuringiensis in a Gram-negative Pseudomonas fluorescens mediated by protoplast fusion. FEMS Microbiol Lett 1994; 122:103–108
    [Google Scholar]
  26. Southwood T.R.E. The insect/plant relationship - an evolutionary perspective. In Insect ¡Plant Relationships 1973 Edited by Van Emden H.F. New York: Wiley; pp 3–29
    [Google Scholar]
  27. Stirpe F., Barbieri L. Ribosome-inactivating proteins up to date. FEBS Lett 1986; 195:1–8
    [Google Scholar]
  28. Tate K.G., Ogawa J.M. Nitidulid beetles as vectors of Monilia fructicola in California stone fruits. Phytopathology 1975; 65:977–983
    [Google Scholar]
  29. Wicklow D.T. Metabolites in the coevolution of fungal chemical defence systems. In Coevolution of Fungi with Plants and Animals 1988 Edited by Pirozynski K.A., Hawksworth D.L. New York: Academic Press; pp 173–201
    [Google Scholar]
  30. Wicklow D.T., Dowd P.F., Tepaske M.R., Gloer J.B. Sclerotial metabolites of Aspergillus fiavus toxic to a detritivorous maize insect. Trans Br My col Soc 1988; 91:433–438
    [Google Scholar]
  31. Wright V.F., Vesonder R.F., Ciegler A. Mycotoxins and other fungal metabolites as insecticides. In Microbial and Viral Pesticides 1982 Edited by Kustak E. New York: Marcel Dekker; pp 559–583
    [Google Scholar]
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