1887

Abstract

A previously described DNA probe specific for (l-141) was fully sequenced and found to consist of 1618 bp and to contain two tandemly repeated ORFs. The deduced amino acid sequence of the two ORFs showed significant homologies with ATP-binding cassette (ABC) transporter proteins, particularly those of the eukaryotic multidrug resistance (MDR) protein family (up to 21 % identity and 47% similarity). A somewhat lower homology was evident with the secretion protein HlyB of the RTX-haemolysin from . The location of the two ORFs on the chromosome was downstream of the gene encoding the 23S rRNA, but transcribed in the opposite direction. PCR amplification and subsequent chromosomal analysis by Southern blot hybridization of several strains showed that all field strains contained the two putative ABC transporter genes. However, some culture collection strains derived from strain J had lost these genes as the result of a 2221 bp deletion.

Loading

Article metrics loading...

/content/journal/micro/10.1099/13500872-142-7-1855
1996-07-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/micro/142/7/mic-142-7-1855.html?itemId=/content/journal/micro/10.1099/13500872-142-7-1855&mimeType=html&fmt=ahah

References

  1. Abiven P., Blanchard B., Saillard C., Kobisch M., Bové J. M. 1992; A specific DNA probe for detecting Mycoplasma hyopneumoniae in experimentally infected piglets. Mol Cell Probes 6:423–429
    [Google Scholar]
  2. Blanchard B., Vena M. M., Cavalier A., Le Lannic J., Gouranton J., Kobisch M. 1992; Electron microscopic observation of the respiratory tract of SPF piglets inoculated with Mycoplasma hyopneumoniae. Vet Microbiol 30:329–341
    [Google Scholar]
  3. Bölske G., Strandberg M. L., Bergström K., Johansson K. E. 1987; Species-specific antigens of Mycoplasma hyopneumoniae and cross-reactions with other porcine mycoplasmas. Curr Microbiol 15:233–239
    [Google Scholar]
  4. Bové J. M. 1993; Molecular features of Mollicutes. Clinical Inf Dis 17:10–31
    [Google Scholar]
  5. Collier A. M., Hu P. C., Clyde W. A. 1985; The changing pathogenicity of Mjcoplasma pneumoniae with passage ‘in vitro’ correlates of virulence. Diagn Microbiol Infect Dis 3:321–326
    [Google Scholar]
  6. Croop J. M. 1993; P-glycoprotein structure and evolutionary homologies. Cytotechnology 12:1–323
    [Google Scholar]
  7. Debey M. C., Ross R. F. 1994; Ciliostatis and loss of cilia induced by Mycoplasma hyopneumoniae in porcine tracheal organ culture. Infect Immun 62:5312–5318
    [Google Scholar]
  8. Dudler R., Schmidhauser C., Parish R. W., Wettenhall R. E., Schmidt T. 1988; A mycoplasma high-affinity transport system and the in vitro invasiveness of mouse sarcoma cells. EMBO J 7:3963–3970
    [Google Scholar]
  9. Fath M., Kolter R. 1993; ABC transporters: bacterial exporters. Microbiol Rev 57:995–1017
    [Google Scholar]
  10. Frey J., Haldimann A., Nicolet J. 1992; Chromosomal heterogeneity of various Mycoplasma hyopneumoniae field strains. Int J Syst Bacteriol 42:275–280
    [Google Scholar]
  11. Gilson G., Alloing G., Schmidt T., Claverys J. P., Dudler R., Hofnung M. 1988; Evidence for high affinity binding-protein dependent transport systems in Gram-positive bacteria and in Mjcoplasma. EMBO J 7:3971–3974
    [Google Scholar]
  12. Gros P., Croop J., Housman D. 1986; Mammalian multidrug resistance gene: complete cDNA sequence indicates strong homology to bacterial transport proteins. Cell 47:371–380
    [Google Scholar]
  13. Hanahan D. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166:557–580
    [Google Scholar]
  14. Higgins C. F. 1992; ABC transporters: from microorganisms to man. Annu Rev Cell Biol 8:67–113
    [Google Scholar]
  15. Higgins C. F., Clyde S. C., Mimmack M. M., Gileadi U., Gill D. R., Gallagher M. P. 1990; Binding protein-dependent transport systems. J Bioenerg Biomembr 22:571–592
    [Google Scholar]
  16. Holland I. B., Kenny B., Blight M. 1990; Haemolysin secretion from E. coli. Biochimie 72:131–141
    [Google Scholar]
  17. Hovind-Hougen K., Friis N. F. 1991; Morphological and ultrastructural studies of Mycoplasma flocculare and Mycoplasma hyopneumoniae in vitro. Res Vet Sci 51:155–163
    [Google Scholar]
  18. Klein C., Kaletta C., Schnell N., Entian K. D. 1992; Analysis of genes involved in biosynthesis of the lantibiotic subtilin. Appl Environ Microbiol 58:132–142
    [Google Scholar]
  19. Kobisch M., Labbé A., Morvan P., Le Moine M. M., Beaurepaire B., Cariolet R., Pansart J. F. 1993; Un modeéle expèrimental associant M. hyopneumoniae et A. pleuropneumoniae. Journée de la Recherche Porcine en France 25:339–344
    [Google Scholar]
  20. L’Ecuyer C., Boulanger P. 1970; Enzootic pneumonia of pigs: identification of a causative mycoplasma in infected pigs and in cultures by immuno-fluorescent staining. Can J Comp Med 34:38–46
    [Google Scholar]
  21. Lincke C. R., Smit J., Velde-Koerts T., Borst P. 1991; Structure of the human MDR 3 gene and physical mapping of the human MDR locus. J Biol Chem 266:5303–5310
    [Google Scholar]
  22. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. J Mol Biol 3:208–218
    [Google Scholar]
  23. Mierendorf R. C., Pfeffer D. 1987; Direct sequencing of denatured plasmid DNA. Methods Enzymol 152:556–562
    [Google Scholar]
  24. Minion C. F., Jarvill-Taylor K. 1994; Membrane-associated hemolysin activities in mycoplasma. FEMS Microbiol Lett 116:101–106
    [Google Scholar]
  25. Ott M. 1993; Dynamics of the bacterial genome: deletions and integrations as mechanisms of bacterial virulence modulation. Zentralbl Bakteriol 278:457–468
    [Google Scholar]
  26. Ross R. F. 1992; Mycoplasmal diseases. In Diseases of Swine, 7th edn. Edited by Leman A. D., Straw B., Mengeling W. L., D’Allaire S., Taylor D. J. Ames: Iowa State University Press; pp 537–551
    [Google Scholar]
  27. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual Cold Spring Harbor, 2nd edn. NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  28. Stemke G. W., Laigret F., Grau O., Bové J. M. 1992; Phylogenetic relationships of three porcine mycoplasmas, Mycoplasma hyopneumoniae, Mycoplasma flocculare, and Mycoplasma hyorhinis, and complete 16S rRNA sequence of M. flocculare. Int J Syst Bacteriol 42:220–225
    [Google Scholar]
  29. Taschke C., Herrmann R. 1986; Analysis of transcription and processing signals of the 16S-23S rRNA operon of Mycoplasma hyopneumoniae. Mol Gen Genet 205:434–441
    [Google Scholar]
  30. Taschke C., Klinkert M. Q., Wolters J., Herrmann R. 1986; Organization of the ribosomal RNA genes in Mycoplasma hyopneumoniae: the 5S rRNA gene is separated from the 16S and 23S rRNA genes. Mol Gen Genet 205:428–433
    [Google Scholar]
  31. Taylor-Robinson D., Furr P. M., Davies H. A. 1981; Mycoplasmal adherence with particular reference to the pathogenicity of Mycoplasma pulmonis. Isr J Med Sci 17:599–603
    [Google Scholar]
  32. Weisburg W. G., Tully J. G., Rose D. L., Petzel J. P., Oyaizu H., Yang D., Mandelco L., Sechrest J., Lawrence T. G., Van Etten J., Maniloff J., Woese G R. 1989; A phylogenetic analysis of the mycoplasmas: basis for their classification. J Bacteriol 171:6455–6467
    [Google Scholar]
  33. Yamao F., Muto A., Kawauchi Y., Iwami M., Iwagami S., Azumi Y., Osawa S. 1985; UGA is read as tryptophan in Mycoplasma capricolum. Proc Natl Acad Sci USA 82:2306–2309
    [Google Scholar]
  34. Zielinsky G., Ross R. F. 1990; Effect of growth in cell cultures and strain on virulence of Mycoplasma hyopneumoniae for swine. Am J Vet Res 51:344–348
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/13500872-142-7-1855
Loading
/content/journal/micro/10.1099/13500872-142-7-1855
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error