@article{mbs:/content/journal/micro/10.1099/13500872-145-3-729, author = "Henriksen, Anne L. Santerre and Even, Sergine and Müller, Christian and Punt, Peter J. and van den Hondel, Cees A. M. J. J. and Nielsen, Jens", title = "Study of the glucoamylase promoter in Aspergillus niger using green fluorescent protein", journal= "Microbiology", year = "1999", volume = "145", number = "3", pages = "729-734", doi = "https://doi.org/10.1099/13500872-145-3-729", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/13500872-145-3-729", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "green fluorescent protein", keywords = "Aspergillus niger", keywords = "glucoamylase promoter", keywords = "chemostat", abstract = "An Aspergillus niger strain expressing a red-shifted green fluorescent protein (GFP) in the cytoplasm under the control of the glucoamylase promoter (PglaA) was characterized with respect to its physiology and morphology. Although xylose acted as a repressor carbon source during batch cultivations, PglaA-driven GFP expression by the glucoamylase promoter could be demonstrated in xylose-limited continuous cultures. In these cultivations, the xylose concentration was therefore too low to cause repression. Transient experiments initiated with a maltose pulse did not further induce red-shifted GFP production in xylose-limited continuous cultures. Maltose induction under conditions of xylose repression was microscopically observed and quantified in a flow-through chamber. Red-shifted GFP was first produced after 5 h induction. Finally the strain was characterized in glucose-limited continuous cultures, and here the area of the mycelium stained with cytoplasmic GFP increased with increasing specific growth rate, indicating that GFP can be used as a marker of cellular activity in this type of cultivation.", }