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Volume 161, Issue 5

Editor's Choice PilB localization correlates with the direction of twitching motility in the cyanobacterium sp. PCC 6803 Free

Abstract

Twitching motility depends on the adhesion of type IV pili (T4P) to a substrate, with cell movement driven by extension and retraction of the pili. The mechanism of twitching motility, and the events that lead to a reversal of direction, are best understood in rod-shaped bacteria such as In the direction of movement depends on the unipolar localization of the pilus extension and retraction motors PilB and PilT to opposite cell poles. Reversal of direction results from relocalization of PilB and PilT. Some cyanobacteria utilize twitching motility for phototaxis. Here, we examine twitching motility in the cyanobacterium sp. PCC 6803, which has a spherical cell shape without obvious polarity. We use a motile sp. PCC 6803 strain expressing a functional GFP-tagged PilB1 protein to show that PilB1 tends to localize in ‘crescents’ adjacent to a specific region of the cytoplasmic membrane. Crescents are more prevalent under the low-light conditions that favour phototactic motility, and the direction of motility strongly correlates with the orientation of the crescent. We conclude that the direction of twitching motility in sp. PCC 6803 is controlled by the localization of the T4P apparatus, as it is in . The PilB1 crescents in the spherical cells of can be regarded as being equivalent to the leading pole in the rod-shaped cells.

  • Received:
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2015-05-01
2024-04-16
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References

  1. Bhaya D., Bianco N. R., Bryant D., Grossman A. 2000; Type IV pilus biogenesis and motility in the cyanobacterium Synechocystis sp. PCC6803. Mol Microbiol 37:941–951 [View Article][PubMed]
     [Google Scholar]
  2. Bhaya D., Takahashi A., Grossman A. R. 2001; Light regulation of type IV pilus-dependent motility by chemosensor-like elements in Synechocystis PCC6803. Proc Natl Acad Sci U S A 98:7540–7545 [View Article][PubMed]
     [Google Scholar]
  3. Bhaya D., Nakasugi K., Fazeli F., Burriesci M. S. 2006; Phototaxis and impaired motility in adenylyl cyclase and cyclase receptor protein mutants of Synechocystis sp. strain PCC 6803. J Bacteriol 188:7306–7310 [View Article][PubMed]
     [Google Scholar]
  4. Bryan S. J., Burroughs N. J., Shevela D., Yu J., Rupprecht E., Liu L.-N., Mastroianni G., Xue Q., Llorente-Garcia I. et al. 2014; Localisation and interactions of the Vipp1 protein in cyanobacteria. Mol Microbiol 94:1179–1195 [View Article][PubMed]
     [Google Scholar]
  5. Bulyha I., Schmidt C., Lenz P., Jakovljevic V., Höne A., Maier B., Hoppert M., Søgaard-Andersen L. 2009; Regulation of the type IV pili molecular machine by dynamic localization of two motor proteins. Mol Microbiol 74:691–706 [View Article][PubMed]
     [Google Scholar]
  6. Burrows L. L. 2012; Pseudomonas aeruginosa twitching motility: type IV pili in action. Annu Rev Microbiol 66:493–520 [View Article][PubMed]
     [Google Scholar]
  7. Chiang P., Habash M., Burrows L. L. 2005; Disparate subcellular localization patterns of Pseudomonas aeruginosa type IV pilus ATPases involved in twitching motility. J Bacteriol 187:829–839 [View Article][PubMed]
     [Google Scholar]
  8. Choi J. S., Chung Y. H., Moon Y. J., Kim C., Watanabe M., Song P. S., Joe C. O., Bogorad L., Park Y. M. 1999; Photomovement of the gliding cyanobacterium Synechocystis sp. PCC 6803. Photochem Photobiol 70:95–102 [View Article][PubMed]
     [Google Scholar]
  9. Fiedler B., Börner T., Wilde A. 2005; Phototaxis in the cyanobacterium Synechocystis sp. PCC 6803: role of different photoreceptors. Photochem Photobiol 81:1481–1488 [View Article][PubMed]
     [Google Scholar]
  10. Gorby Y. A., Yanina S., McLean J. S., Rosso K. M., Moyles D., Dohnalkova A., Beveridge T. J., Chang I. S., Kim B. H. et al. 2006; Electrically conductive bacterial nanowires produced by Shewanella oneidensis strain MR-1 and other microorganisms. Proc Natl Acad Sci U S A 103:11358–11363 [View Article][PubMed]
     [Google Scholar]
  11. Jakovljevic V., Leonardy S., Hoppert M., Søgaard-Andersen L. 2008; PilB and PilT are ATPases acting antagonistically in type IV pilus function in Myxococcus xanthus. . J Bacteriol 190:2411–2421 [View Article][PubMed]
     [Google Scholar]
  12. Kuchmina E., Wallner T., Kryazhov S., Zinchenko V. V., Wilde A. 2012; An expression system for regulated protein production in Synechocystis sp PCC 6803 and its application for construction of a conditional knockout of the ferrochelatase enzyme. J Biotechnol 16275–80
     [Google Scholar]
  13. Liberton M., Berg R. H., Heuser J., Roth R., Pakrasi H. B. 2006; Ultrastructure of the membrane systems in the unicellular cyanobacterium Synechocystis sp. strain PCC 6803. Protoplasma 227:129–138 [View Article][PubMed]
     [Google Scholar]
  14. Linhartová M., Bučinská L., Halada P., Ječmen T., Setlík J., Komenda J., Sobotka R. 2014; Accumulation of the type IV prepilin triggers degradation of SecY and YidC and inhibits synthesis of photosystem II proteins in the cyanobacterium Synechocystis PCC 6803. Mol Microbiol 93:1207–1223[PubMed]
     [Google Scholar]
  15. Mattick J. S. 2002; Type IV pili and twitching motility. Annu Rev Microbiol 56:289–314 [View Article][PubMed]
     [Google Scholar]
  16. Ng W.-O., Grossman A. R., Bhaya D. 2003; Multiple light inputs control phototaxis in Synechocystis sp. strain PCC6803. J Bacteriol 185:1599–1607 [View Article][PubMed]
     [Google Scholar]
  17. Okajima K., Yoshihara S., Fukushima Y., Geng X., Katayama M., Higashi S., Watanabe M., Sato S., Tabata S. et al. 2005; Biochemical and functional characterization of BLUF-type flavin-binding proteins of two species of cyanobacteria. J Biochem 137:741–750 [View Article][PubMed]
     [Google Scholar]
  18. Peabody C. R., Chung Y. J., Yen M.-R., Vidal-Ingigliardi D., Pugsley A. P., Saier M. H. Jr 2003; Type II protein secretion and its relationship to bacterial type IV pili and archaeal flagella. Microbiology 149:3051–3072 [View Article][PubMed]
     [Google Scholar]
  19. Pelicic V. 2008; Type IV pili: e pluribus unum?. Mol Microbiol 68:827–837 [View Article][PubMed]
     [Google Scholar]
  20. Rippka R., Deruelles J., Waterbury J. B., Herdman M., Stanier R. Y. 1979; Generic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61 [View Article]
     [Google Scholar]
  21. Schuergers N., Ruppert U., Watanabe S., Nürnberg D. J., Lochnit G., Dienst D., Mullineaux C. W., Wilde A. 2014; Binding of the RNA chaperone Hfq to the type IV pilus base is crucial for its function in Synechocystis sp. PCC 6803. Mol Microbiol 92:840–852 [View Article][PubMed]
     [Google Scholar]
  22. Song J.-Y., Cho H. S., Cho J.-I., Jeon J.-S., Lagarias J. C., Park Y.-I. 2011; Near-UV cyanobacteriochrome signaling system elicits negative phototaxis in the cyanobacterium Synechocystis sp. PCC 6803. Proc Natl Acad Sci U S A 108:10780–10785 [View Article][PubMed]
     [Google Scholar]
  23. Wadhams G. H., Armitage J. P. 2004; Making sense of it all: bacterial chemotaxis. Nat Rev Mol Cell Biol 5:1024–1037 [View Article][PubMed]
     [Google Scholar]
  24. Wilde A., Fiedler B., Börner T. 2002; The cyanobacterial phytochrome Cph2 inhibits phototaxis towards blue light. Mol Microbiol 44:981–988 [View Article][PubMed]
     [Google Scholar]
  25. Yoshihara S., Ikeuchi M. 2004; Phototactic motility in the unicellular cyanobacterium Synechocystis sp. PCC 6803. Photochem Photobiol Sci 3:512–518 [View Article][PubMed]
     [Google Scholar]
  26. Yoshihara S., Suzuki F., Fujita H., Geng X. X., Ikeuchi M. 2000; Novel putative photoreceptor and regulatory genes required for the positive phototactic movement of the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol 41:1299–1304 [View Article][PubMed]
     [Google Scholar]
  27. Yoshihara S., Geng X., Okamoto S., Yura K., Murata T., Go M., Ohmori M., Ikeuchi M. 2001; Mutational analysis of genes involved in pilus structure, motility and transformation competency in the unicellular motile cyanobacterium Synechocystis sp. PCC 6803. Plant Cell Physiol 42:63–73 [View Article][PubMed]
     [Google Scholar]
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PilB localization correlates with the direction of twitching motility in the cyanobacterium Synechocystis sp. PCC 6803
Microbiology 161, 960 (2015); https://doi.org/10.1099/mic.0.000064
/content/journal/micro/10.1099/mic.0.000064
/content/journal/micro/10.1099/mic.0.000064
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