RT Journal Article SR Electronic(1) A1 Kopecká, Marie A1 Yamaguchi, Masashi A1 Kawamoto, SusumuYR 2015 T1 Effects of the F-actin inhibitor latrunculin A on the budding yeast Saccharomyces cerevisiae JF Microbiology, VO 161 IS 7 SP 1348 OP 1355 DO https://doi.org/10.1099/mic.0.000091 PB Microbiology Society, SN 1465-2080, AB Our basic cell biology research was aimed at investigating the effect on eukaryotic cells of the sudden loss of the F-actin cytoskeleton. Cells treated with latrunculin A (LA) in yeast extract peptone dextrose (YEPD) medium were examined using phase-contrast and fluorescent microscopy, freeze-substitution, transmission and scanning electron microscopy, counted using a Bürker chamber and their absorbance measured. The cells responded to the presence of LA, an F-actin inhibitor, with the disappearance of actin patches, actin cables and actin rings. This resulted in the formation of larger spherical cells with irregular morphology in the cell walls and ultrastructural disorder of the cell organelles and secretory vesicles. Instead of buds, LA-inhibited cells formed only ‘table-mountain-like’ wide flattened swellings without apical growth with a thinner glucan cell-wall layer containing β-1,3-glucan microfibrils. The LA-inhibited cells lysed. Actin cables and patches were required for bud formation and bud growth. In addition, actin patches were required for the formation of β-1,3-glucan microfibrils in the bud cell wall. LA has fungistatic, fungicidal and fungilytic effects on the budding yeast Saccharomyces cerevisiae., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000091