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Volume 161, Issue 8

The lysis cassette of DLP12 defective prophage is regulated by RpoE Free

Abstract

Expression of the lysis cassette () from the defective lambdoid prophage at the 12th minute of genome (DLP12) is required in some strains for proper curli expression and biofilm formation. Regulating production of the lytic enzymes encoded by these genes is critical for maintaining cell wall integrity. In lambdoid phages, late-gene regulation is mediated by the vegetative sigma factor RpoD and the lambda antiterminator Q. We previously demonstrated that DLP12 contains a Q-like protein (Q) that positively regulates transcription of the lysis cassette, but the sigma factor responsible for this transcription initiation remained to be elucidated. analysis of revealed the presence of a putative − 35 and − 10 sigma site recognized by the extracytoplasmic stress response sigma factor, RpoE. In this work, we report that RpoE overexpression promoted transcription from and using purified RNAP. We demonstrate that the − 35 region is important for RpoE binding and that this region is also important for Q-mediated transcription of . A bacterial two-hybrid assay indicated that Q and RpoE physically interact , consistent with what is seen for Q and RpoD. We propose that RpoE regulates transcription of the DLP12 lysis genes through interaction with Q and that proper expression is dependent on an intact − 35 sigma region in . This work provides evidence that the unique Q-dependent regulatory mechanism of lambdoid phages has been co-opted by harbouring defective DLP12 and has been integrated into the tightly controlled RpoE regulon.

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2015-08-01
2024-04-18
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The lysis cassette of DLP12 defective prophage is regulated by RpoE
Microbiology 161, 1683 (2015); https://doi.org/10.1099/mic.0.000115
/content/journal/micro/10.1099/mic.0.000115
/content/journal/micro/10.1099/mic.0.000115
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