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f Roles of the TonB1 and TonB2 proteins in haemin iron acquisition and virulence in

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Roles of the TonB1 and TonB2 proteins in haemin iron acquisition and virulence in , Page 1 of 1

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  • Two TonB systems in were found and characterized as ExbB1–ExbD1–TonB1 and ExbB2–ExbD2–ExbD2′–TonB2, but the significance of two sets of TonB complexes in is not clear. In this study, by deleting the or gene of strain CH3, we investigated the roles of the TonB1 and TonB2 proteins in iron acquisition and virulence. The results showed that strain CH3 could utilize haemin as the sole iron source in the presence of -cysteine, but haemin iron acquisition was defective in the CH3Δ mutant, and the deletion of either or significantly reduced adhesion to and invasion of Vero cells. Animal experiments indicated that the LD of the CH3Δ and CH3Δ mutants in ducklings was ∼224- and ∼87-fold, respectively, higher than that of the WT CH3 strain. Additional analysis indicated that blood bacterial loading of ducklings infected with CH3Δ or CH3Δ decreased significantly compared with that found for WT CH3-infected ducklings. Thus, our results indicated that the TonB1, but not TonB2 protein, is involved in haemin iron acquisition and that both TonB proteins are necessary for optimal bacterial virulence.

  • The GenBank/EMBL/DDBJ accession numbers for the sequences of the TonB1 and TonB2 systems in strain CH3 are KM393215 and KM393216, respectively.

  • One supplementary table and two supplementary figures are available with the online Supplementary Material.

  • D. Nies

  • Abbreviation:
    DIP 2,2′-dipyridyl

© 2015 The Authors

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2015-08-01
2019-02-22
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