@article{mbs:/content/journal/micro/10.1099/mic.0.000155, author = "Cullen, Louise and Weiser, Rebecca and Olszak, Tomasz and Maldonado, Rita F. and Moreira, Ana S. and Slachmuylders, Lisa and Brackman, Gilles and Paunova-Krasteva, Tsvetelina S. and Zarnowiec, Paulina and Czerwonka, Grzegorz and Reilly, James and Drevinek, Pavel and Kaca, Wieslaw and Melter, Oto and De Soyza, Anthony and Perry, Audrey and Winstanley, Craig and Stoitsova, Stoyanka R. and Lavigne, Rob and Mahenthiralingam, Eshwar and Sá-Correia, Isabel and Coenye, Tom and Drulis-Kawa, Zuzanna and Augustyniak, Daria and Valvano, Miguel A. and McClean, Siobhán", title = "Phenotypic characterization of an international Pseudomonas aeruginosa reference panel: strains of cystic fibrosis (CF) origin show less in vivo virulence than non-CF strains", journal= "Microbiology", year = "2015", volume = "161", number = "10", pages = "1961-1977", doi = "https://doi.org/10.1099/mic.0.000155", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000155", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Pseudomonas aeruginosa causes chronic lung infections in people with cystic fibrosis (CF) and acute opportunistic infections in people without CF. Forty-two P. aeruginosa strains from a range of clinical and environmental sources were collated into a single reference strain panel to harmonise research on this diverse opportunistic pathogen. To facilitate further harmonized and comparable research on P. aeruginosa, we characterized the panel strains for growth rates, motility, virulence in the Galleria mellonella infection model, pyocyanin and alginate production, mucoid phenotype, LPS pattern, biofilm formation, urease activity, and antimicrobial and phage susceptibilities. Phenotypic diversity across the P. aeruginosa panel was apparent for all phenotypes examined, agreeing with the marked variability seen in this species. However, except for growth rate, the phenotypic diversity among strains from CF versus non-CF sources was comparable. CF strains were less virulent in the G. mellonella model than non-CF strains (P = 0.037). Transmissible CF strains generally lacked O-antigen, produced less pyocyanin and had low virulence in G. mellonella. Furthermore, in the three sets of sequential CF strains, virulence, O-antigen expression and pyocyanin production were higher in the earlier isolate compared to the isolate obtained later in infection. Overall, this full phenotypic characterization of the defined panel of P. aeruginosa strains increases our understanding of the virulence and pathogenesis of P. aeruginosa and may provide a valuable resource for the testing of novel therapies against this problematic pathogen.", }