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f An iso-15 : 0 -alkylglycerol moiety is the key structure of the E-signal in

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  • Authors: Tilman Ahrendt1​,3 ,  Christina Dauth1​,3 , Helge B. Bode1​,2​
    • VIEW AFFILIATIONS
    • 1​ 1​Merck Stiftungsprofessur für Molekulare Biotechnologie, Fachbereich Biowissenschaften, Max-von-Laue-Strasse 9, 60438 Frankfurt am Main, Germany 2​ 2​Buchmann Institute for Molecular Life Sciences (BMLS), Goethe Universität Frankfurt, Max-von-Laue-Strasse 15, 60438 Frankfurt am Main, Germany
  • Helge B. Bode [email protected]
  • First Published Online: 01 January 2016, Microbiology 162: 138-144, doi: 10.1099/mic.0.000169
  • Subject: Physiology and Metabolism
  • Received:
  • Accepted:
  • Cover date:
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An iso-15 : 0 -alkylglycerol moiety is the key structure of the E-signal in , Page 1 of 1

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  • The E-signal is one of five intercellular signals (named A- to E-signal) guiding fruiting body development in , and it has been shown to be a combination of the branched-chain fatty acid (FA) iso-15 : 0 and the diacylmonoalkyl ether lipid TG1. Developmental mutants HB015 (Δ MXAN_4265::) and (MXAN_1528::) are blocked at different stages of fruiting body and spore formation as they cannot form the required iso-FA or the actual ether lipid, respectively. In order to define the structural basis of the E-signal, different mono- and triglycerides containing ether or ester bonds were synthesized and used for complementation of these mutants. Here, the monoalkylglyceride -1--(13-methyltetradecyl)glycerol exhibited comparably high levels of complementation in both mutants, restoring fruiting body and spore formation, identifying iso-15 : 0 -alkylglycerol, part of the natural lipid TG1, as the ‘signalophore’ of E-signalling.

  • These authors contributed equally to this work.

  • Supplementary Methods and 29 supplementary figures are available with the online Supplementary Material.

  • Edited by: F. Sargent

  • Abbreviations:
    FA fatty acid
    TG1 1-iso-15 : 0-alkyl-2,3-di-iso-15 : 0 acyl glycerol.

© 2016 The Authors

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2016-01-01
2019-02-15
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