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Volume 162, Issue 7

Developmental delay in a null mutant is associated with the accumulation of -maltose 1-phosphate Open Access

Abstract

The GlgE pathway is thought to be responsible for the conversion of trehalose into a glycogen-like -glucan polymer in bacteria. Trehalose is first converted to maltose, which is phosphorylated by maltose kinase Pep2 to give -maltose 1-phosphate. This is the donor substrate of the maltosyl transferase GlgE that is known to extend -1,4-linked maltooligosaccharides, which are thought to be branched with -1,6 linkages. The genome of contains all the genes coding for the GlgE pathway enzymes but none of those of related pathways, including and of the glycogen pathway. This provides an opportunity to study the GlgE pathway in isolation. The genes of the GlgE pathway were upregulated at the onset of sporulation, consistent with the known timing of -glucan deposition. A constructed Δ null mutant strain was viable but showed a delayed developmental phenotype when grown on maltose, giving less cell mass and delayed sporulation. Pre-spore cells and spores of the mutant were frequently double the length of those of the wild-type, implying impaired cross-wall formation, and spores showed reduced tolerance to stress. The mutant accumulated -maltose 1-phosphate and maltose but no -glucan. Therefore, the GlgE pathway is necessary and sufficient for polymer biosynthesis. Growth of the Δ mutant on galactose and that of a Δ mutant on maltose were analysed. In both cases, neither accumulation of -maltose 1-phosphate/-glucan nor a developmental delay was observed. Thus, high levels of -maltose 1-phosphate are responsible for the developmental phenotype of the Δ mutant, rather than the lack of -glucan.

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Keyword(s): GlgE , glucan , glycogen , polysaccharide , sporulation and Streptomyces
© 2016 The Authors
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2016-07-01
2024-04-16
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Developmental delay in a Streptomyces venezuelae glgE null mutant is associated with the accumulation of α-maltose 1-phosphate
Microbiology 162, 1208 (2016); https://doi.org/10.1099/mic.0.000296
/content/journal/micro/10.1099/mic.0.000296
/content/journal/micro/10.1099/mic.0.000296
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