RT Journal Article SR Electronic(1) A1 Jacob, Stefan A1 Grötsch, Thomas A1 Foster, Andrew J A1 Schüffler, Anja A1 Rieger, Patrick H A1 Sandjo, Louis P A1 Liermann, Johannes C A1 Opatz, Till A1 Thines, EckhardYR 2017 T1 Unravelling the biosynthesis of pyriculol in the rice blast fungus Magnaporthe oryzae JF Microbiology, VO 163 IS 4 SP 541 OP 553 DO https://doi.org/10.1099/mic.0.000396 PB Microbiology Society, SN 1465-2080, AB Pyriculol was isolated from the rice blast fungus Magnaporthe oryzae and found to induce lesion formation on rice leaves. These findings suggest that it could be involved in virulence. The gene MoPKS19 was identified to encode a polyketide synthase essential for the production of the polyketide pyriculol in the rice blast fungus M. oryzae. The transcript abundance of MoPKS19 correlates with the biosynthesis rate of pyriculol in a time-dependent manner. Furthermore, gene inactivation of MoPKS19 resulted in a mutant unable to produce pyriculol, pyriculariol and their dihydro derivatives. Inactivation of a putative oxidase-encoding gene MoC19OXR1, which was found to be located in the genome close to MoPKS19, resulted in a mutant exclusively producing dihydropyriculol and dihydropyriculariol. By contrast, overexpression of MoC19OXR1 resulted in a mutant strain only producing pyriculol. The MoPKS19 cluster, furthermore, comprises two transcription factors MoC19TRF1 and MoC19TRF2, which were both found individually to act as negative regulators repressing gene expression of MoPKS19. Additionally, extracts of ΔMopks19 and ΔMoC19oxr1 made from axenic cultures failed to induce lesions on rice leaves compared to extracts of the wild-type strain. Consequently, pyriculol and its isomer pyriculariol appear to be the only lesion-inducing secondary metabolites produced by M. oryzae wild-type (MoWT) under these culture conditions. Interestingly, the mutants unable to produce pyriculol and pyriculariol were as pathogenic as MoWT, demonstrating that pyriculol is not required for infection., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000396