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Volume 163, Issue 5

High intracellular c-di-GMP levels antagonize quorum sensing and virulence gene expression in H111 Free

Abstract

The opportunistic human pathogen H111 uses two chemically distinct signal molecules for controlling gene expression in a cell density-dependent manner: -acyl-homoserine lactones (AHLs) and -2-dodecenoic acid (BDSF). Binding of BDSF to its cognate receptor RpfR lowers the intracellular c-di-GMP level, which in turn leads to differential expression of target genes. In this study we analysed the transcriptional profile of H111 upon artificially altering the cellular c-di-GMP level. One hundred and eleven genes were shown to be differentially expressed, 96 of which were downregulated at a high c-di-GMP concentration. Our analysis revealed that the BDSF, AHL and c-di-GMP regulons overlap for the regulation of 24 genes and that a high c-di-GMP level suppresses expression of AHL-regulated genes. Phenotypic analyses confirmed changes in the expression of virulence factors, the production of AHL signal molecules and the biosynthesis of different biofilm matrix components upon altered c-di-GMP levels. We also demonstrate that the intracellular c-di-GMP level determines the virulence of to and .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Burkholderia and c-di-GMP
© 2017 The Authors
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2017-05-01
2024-04-23
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High intracellular c-di-GMP levels antagonize quorum sensing and virulence gene expression in Burkholderia cenocepacia H111
Microbiology 163, 754 (2017); https://doi.org/10.1099/mic.0.000452
/content/journal/micro/10.1099/mic.0.000452
/content/journal/micro/10.1099/mic.0.000452
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