Post-transcriptional regulation of target genes by the sRNA FnrS in Neisseria gonorrhoeae
Tanwer, Pooja and Bauer, Susanne and Heinrichs, Elisabeth and Panda, Gurudutta and Saluja, Daman and Rudel, Thomas and Beier, Dagmar,, 163, 1081-1092 (2017),
doi = https://doi.org/10.1099/mic.0.000484,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Small non-coding RNAs (sRNAs) are well-established post-transcriptional regulators of gene expression in bacteria that respond to a variety of environmental stimuli. They usually act by base-pairing with their target mRNAs, which is commonly facilitated by the RNA chaperone Hfq. In this study we initiated the analysis of the sRNA FnrS of Neisseria gonorrhoeae, which is induced under anaerobic conditions. We identified four putative FnrS target genes using bioinformatics approaches and validated these target genes using translational reporter gene fusions in both Escherichia coli and N. gonorrhoeae, thereby demonstrating their downregulation by direct base-pairing between the respective mRNA and FnrS. We demonstrate deregulation of target mRNAs upon deletion of fnrS and provide evidence that the isc gene cluster required for iron–sulfur cluster biosynthesis, which harbours iscS, which is a direct target of FnrS, is coordinately downregulated by the sRNA. By mutational analysis we show that, surprisingly, three distinct regions of FnrS are employed for interaction with different target genes.,
language=,
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