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Volume 163, Issue 7

produces indole-3-acetic acid, which promotes root growth in and enhances virulence to insects Free

Abstract

The plant root colonizing insect-pathogenic fungus has been shown to boost plant growth, but little is known about the responsible mechanisms. Here we show that promotes lateral root growth and root hair development of seedlings in part through an auxin [indole-3-acetic acid (IAA)]-dependent mechanism. , or its auxin-containing culture filtrate promoted root proliferation, activated IAA-regulated gene expression and rescued the root hair defect of the IAA-deficient mutant. Substrate feeding assays suggest that possesses tryptamine (TAM) and indole-3-acetamide tryptophan (Trp)-dependent auxin biosynthetic pathways. Deletion of impaired IAA production by blocking conversion of Trp to TAM but the reduction was not sufficient to affect plant growth enhancement. We also show that secretes IAA on insect cuticle. ∆ produced fewer infection structures and was less virulent to insects than the wild-type, whereas spores harvested from culture media containing IAA were more virulent. Furthermore, exogenous application of IAA increased appressorial formation and virulence. Together, these results suggest that auxins play an important role in the ability of to promote plant growth, and the endogenous pathways for IAA production may also be involved in regulating entomopathogenicity. Auxins were also produced by other species and the endophytic insect pathogen suggesting that interplay between plant- and fungal-derived auxins has important implications for plant–microbe–insect interactions.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): auxin , interaction , Metarhizium , plant-associated microbes and virulence
© 2017 The Authors
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2017-07-01
2024-04-19
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Metarhizium robertsii produces indole-3-acetic acid, which promotes root growth in Arabidopsis and enhances virulence to insects
Microbiology 163, 980 (2017); https://doi.org/10.1099/mic.0.000494
/content/journal/micro/10.1099/mic.0.000494
/content/journal/micro/10.1099/mic.0.000494
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