1887

Abstract

The aim of the study was to investigate the neutralizing effect of lactobacilli isolated from indigenous food and human sources on enteropathogenic (EPEC) O26 : H11-induced epithelial barrier dysfunction . This was assessed by transepithelial electrical resistance (TEER) and permeability assays using intestinal cell lines, HT-29 and Caco-2. Furthermore, the expression and distribution of tight junction (TJ) proteins were analysed by qRT-PCR and immunofluorescence assay, respectively. The nine strains used in the study were from different species viz. , , salivarius and plantarum. All strains were able to reverse the decrease in TEER and corresponding increase in permeability across infected monolayers. Maximum reversal was observed after 18 h [up to 93.8±2.0 % by GG followed by IIs11.2 (92.6±2.2 %) and GRI-2 (91.9±0.9 %)] of lactobacilli exposure following EPEC O26 : H11 infection. All strains were able to redistribute the TJ proteins to the cell periphery either partially or completely. Moreover, FA-7 was also able to significantly increase the mRNA expression of ZO-1 and claudin-1 (2.5-fold and 3.0-fold, respectively; <0.05). The rapid reversal observed by these strains could be mostly because of the redistribution rather than increased mRNA expression of TJ proteins. In conclusion, FA-7, FA-1 and GRI-2 were good in all the aspects studied, and the other strains were good in some aspects. FA-7, FA-1 and GRI-2 can therefore be used for potential therapeutic purpose against intestinal epithelial dysfunction.

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2017-09-01
2024-03-29
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