@article{mbs:/content/journal/micro/10.1099/mic.0.000561, author = "Mukherjee, Sandipan and Ramesh, Aiyagari", title = "Dual-label flow cytometry-based host cell adhesion assay to ascertain the prospect of probiotic Lactobacillus plantarum in niche-specific antibacterial therapy", journal= "Microbiology", year = "2017", volume = "163", number = "12", pages = "1822-1834", doi = "https://doi.org/10.1099/mic.0.000561", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000561", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "probiotic", keywords = "Lactobacillus plantarum", keywords = "bacteriocin", keywords = "flow cytometry", keywords = "adhesion", abstract = "Host cell adhesion assays that provide quantitative insight on the potential of lactic acid bacteria (LAB) to inhibit adhesion of intestinal pathogens can be leveraged for the development of niche-specific anti-adhesion therapy. Herein, we report a dual-colour flow cytometry (FCM) analysis to assess the ability of probiotic Lactobacillus plantarum strains to impede adhesion of Enterococcus faecalis, Listeria monocytogenes and Staphylococcus aureus onto HT-29 cells. FCM in conjunction with a hierarchical cluster analysis could discern the anti-adhesion potential of L. plantarum strains, wherein the efficacy of L. plantarum DF9 was on a par with the probiotic L. rhamnosus GG. Combination of FCM with principal component analysis illustrated the relative influence of LAB strains on adhesion parameters k d and e m of the pathogen and identified probiotic LAB suitable for anti-adhesion intervention. The analytical merit of the FCM analysis was captured in host cell adhesion assays that measured relative elimination of adhered LAB vis-à-vis pathogens, on exposure to either LAB bacteriocins or therapeutic antibiotics. It is envisaged that the dual-colour FCM-based adhesion assay described herein would enable a fundamental understanding of the host cell adhesion process and stimulate interest in probiotic LAB as safe anti-adhesion therapeutic agents against gastrointestinal pathogens.", }