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f Ethylene production in Synechocystis sp. PCC 6803 promotes phototactic movement

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  • Authors: Ekaterina Kuchmina1​ ,  Stephan Klähn1​ ,  Annik Jakob1​,2​ ,  Werner Bigott1​ ,  Heike Enke3​,†​ ,  Ulf Dühring3​ , Annegret Wilde1​,4​
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    • 1​ 1​Institute of Biology III, University of Freiburg, Schänzlestraße 1, 79104 Freiburg, Germany 2​ 2​Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany 3​ 3​Algenol Biofuels Germany GmbH, Magnusstraße 11, 12489 Berlin, Germany 4​ 4​BIOSS Centre for Biological Signalling Studies, University of Freiburg, 79104 Freiburg, Germany †​ †​Present address: Cyano Biotech GmbH, Magnusstraße 11, 12489 Berlin, Germany.
  • *Correspondence: Annegret Wilde [email protected]
  • First Published Online: 01 November 2017, Microbiology 163: 1937-1945, doi: 10.1099/mic.0.000564
  • Subject: Regulation
  • Received:
  • Accepted:
  • Cover date:
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Ethylene production in Synechocystis sp. PCC 6803 promotes phototactic movement, Page 1 of 1

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  • Ethylene is a gaseous signal sensed by plants and bacteria. Heterologous expression of the ethylene-forming enzyme (EFE) from Pseudomonas syringae in cyanobacteria leads to the production of ethylene under photoautotrophic conditions. The recent characterization of an ethylene-responsive signalling pathway affecting phototaxis in the cyanobacterium Synechocystis sp. PCC 6803 implied that biotechnologically relevant ethylene synthesis may induce regulatory processes that are not related to changes in metabolism. Here, we provide data that indicate that endogenously produced ethylene accelerates the movement of cells towards light. Microarray analysis demonstrates that ethylene mainly deactivates transcription from the csiR1/lsiR promoter, which is under the control of the two-component system consisting of the ethylene- and UV-A-sensing histidine kinase UirS and the DNA-binding response regulator UirR. Surprisingly, ethylene production triggers a very specific transcriptional response and only a few other smaller transcriptional changes are detected in the microarray analysis.

  • Three supplementary figures, two supplementary tables and one data file are available with the online version of this article.

© 2017 The Authors

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2019-02-16
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