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Volume 164, Issue 1

ParA-like protein influences the distribution of multi-copy chromosomes in cyanobacterium PCC 7942 Free

Abstract

While many bacteria, such as and , harbour a single-copy chromosome, freshwater cyanobacteria have multiple copies of each chromosome per cell. Although it has been reported that multi-copy chromosomes are evenly distributed along the major axis of the cell in cyanobacterium PCC 7942, the distribution mechanism of these chromosomes remains unclear. In , the carboxysome, a metabolic microcompartment for carbon fixation that is distributed in a similar manner to the multi-copy chromosomes, is regulated by ParA-like protein (hereafter ParA). To elucidate the role of ParA in the distribution of multi-copy chromosomes, we constructed and analysed ParA disruptant and overexpressing strains of . Our fluorescence hybridization assay revealed that the disruptants displayed an aberrant distribution of their multi-copy chromosomes. In the disruptant the multiple origin and terminus foci, corresponding to the intracellular position of each chromosomal region, were aggregated, which was compensated by the expression of exogenous ParA from other genomic loci. The disruptant is sensitive to UV-C compared to the WT strain. Additionally, giant cells appeared under ParA overexpression at the late stage of growth indicating that excess ParA indirectly inhibits cell division. Screening of the ParA-interacting proteins by yeast two-hybrid analysis revealed four candidates that are involved in DNA repair and cell membrane biogenesis. These results suggest that ParA is involved in the pleiotropic cellular functions with these proteins, while is dispensable for cell viability in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): cell division , chromosomal distribution , cyanobacteria and cytoskeleton
© 2018 The Authors
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2018-01-01
2024-04-20
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ParA-like protein influences the distribution of multi-copy chromosomes in cyanobacterium Synechococcus elongatus PCC 7942
Microbiology 164, 45 (2018); https://doi.org/10.1099/mic.0.000577
/content/journal/micro/10.1099/mic.0.000577
/content/journal/micro/10.1099/mic.0.000577
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