RT Journal Article SR Electronic(1) A1 Hashimoto, Masayuki A1 Matsushima, Hiroaki A1 Suparthana, I. Putu A1 Ogasawara, Hiroshi A1 Yamamoto, Hiroki A1 Teng, ChingHao A1 Sekiguchi, JunichiYR 2018 T1 Digestion of peptidoglycan near the cross-link is necessary for the growth of Bacillus subtilis JF Microbiology, VO 164 IS 3 SP 299 OP 307 DO https://doi.org/10.1099/mic.0.000614 PB Microbiology Society, SN 1465-2080, AB Bacterial cells are covered with peptidoglycan (PG) layer(s), serving as the cellular exoskeleton. The PG sacculus changes its shape during cell growth, and thus both the synthesis and disassembly of PG are important for cell proliferation. In Bacillus subtilis, four dl-endopeptidases (DLEPases; LytE, LytF, CwlO and CwlS) are involved in the maintenance of cell morphology. The lytE cwlO double mutant exhibits synthetic lethality and defective cell elongation, while the lytE lytF cwlS triple mutant exhibits defective cell separation, albeit with septum formation. LytE is involved in both cell separation and elongation. We propose that DLEPases have varied roles in cell separation and elongation. To determine these roles, the catalytic domain of LytE was substituted with another catalytic domain that digests the other bonds in PG. By using the chimeric enzymes, we assessed the suppression of the synthetic lethality by the cell elongation defect and the disruption of chain morphology by the cell separation defect. All the constructed chimeric enzymes suppressed the cell separation defect, restoring the chain morphology. Digestion at any position of PG broke the linkage between two daughter cells, releasing them from each other. However, only d,d-endopeptidases suppressed the lack of DLEPase in the lytE cwlO double mutant. This indicated that the release of tension on the expanding PG sacculus is not the sole essential function of DLEPases. Considering that the structure of the digested PG is important for cell elongation, the digested product might be reused in the growth process in some way., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000614