1887

Abstract

produces hydrogen sulfide (HS) from -cysteine. However, the role of HS produced by in periodontal inflammation is unclear. In this study, we identified the enzyme that catalyses HS production from -cysteine and analysed the role of HS using a mouse abscess model. The enzyme identified was identical to methionine γ-lyase (PG0343), which produces methyl mercaptan (CHSH) from -methionine. Therefore, we analysed HS and CHSH production by W83 and a PG0343-deletion mutant (ΔPG0343) with/without -cysteine and/or -methionine. The results indicated that CHSH is produced constitutively irrespective of the presence of -methionine, while HS was greatly increased by both W83 and ΔPG0343 in the presence of -cysteine. In contrast, CHSH production by ΔPG0343 was absent irrespective of the presence of -methionine, and HS production was eliminated in the absence of -cysteine. Thus, CHSH and HS production involves different substrates, -methionine or -cysteine, respectively. Based on these characteristics, we analysed the roles of CHSH and HS in abscess formation in mice by W83 and ΔPG0343. Abscess formation by W83, but not ΔPG0343, differed significantly in the presence and absence of -cysteine. In addition, the presence of -methionine did not affect the size of abscesses generated by W83 and ΔPG0343. Therefore, we conclude that HS produced by does not induce inflammation; however, HS enhances inflammation caused by CHSH. Thus, these results suggest the HS produced by plays a supportive role in inflammation caused by methionine γ-lyase.

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2018-04-01
2024-03-28
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