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Volume 164, Issue 4

Hydrophobins contribute to root colonization and stress responses in the rhizosphere-competent insect pathogenic fungus Free

Abstract

The hyd1/hyd2 hydrophobins are important constituents of the conidial cell wall of the insect pathogenic fungus . This fungus can also form intimate associations with several plant species. Here, we show that inactivation of two Class I hydrophobin genes, or significantly decreases the interaction of with bean roots. Curiously, the double mutant was less impaired in root association than or . Loss of genes affected growth rate, conidiation ability and oosporein production. Expression patterns for genes involved in conidiation, cell wall integrity, insect virulence, signal transduction, adhesion, hydrophobicity and oosporein production were screened in the deletion mutants grown in different conditions. Repression of the major MAP-Kinase signal transduction pathways ( MAPK pathway) was observed that was more pronounced in the single versus double mutants under certain conditions. The double mutant showed up-regulation of the and the transcription factor under certain conditions when compared to the wild-type or single mutants. The expression of the adhesin and the oosporein polyketide synthase 9 gene was severely reduced in all of the mutants. On the other hand, fewer changes were observed in the expression of key conidiation and cell wall integrity genes in mutants compared to wild-type. Taken together, the data from this study indicated pleiotropic consequences of deletion of and on signalling and stress pathways as well as the ability of the fungus to form stable associations with plant roots.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): Beauveria bassiana , entomopathogen , fungal endophyte and hydrophobin
© 2018 The Authors
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2018-04-01
2024-04-18
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Hydrophobins contribute to root colonization and stress responses in the rhizosphere-competent insect pathogenic fungus Beauveria bassiana
Microbiology 164, 517 (2018); https://doi.org/10.1099/mic.0.000644
/content/journal/micro/10.1099/mic.0.000644
/content/journal/micro/10.1099/mic.0.000644
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