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Volume 164, Issue 4

Transcriptome analysis of a -glycerol-3-phosphate dehydrogenase mutant reveals a disruption in bioenergetics Free

Abstract

causes acute and chronic human infections and is the major cause of morbidity and mortality in cystic fibrosis (CF) patients. We previously determined that the glycerol-3-phosphate dehydrogenase encoded by plays a larger role in physiology beyond its role in glycerol metabolism. To better understand the effect of a mutation on physiology we compared the transcriptomes of strain PAO1 and the PAO1Δ mutant using RNA-seq analysis. We determined that a null mutation of significantly altered amino acid metabolism in and affected the production of intermediates that are channelled into the tricarboxylic acid cycle. Moreover, the loss of induced a general stress response mediated by RpoS in Several other phenotypes observed for the mutant include increased persister cell formation, reduced extracellular ATP accumulation and increased heat output. Taken together, these findings implicate glycerol-3-phosphate dehydrogenase as a key player in energy metabolism in .

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): bioenergetics , glycerol , persister cells , Pseudomonas aeruginosa , RpoS and transcriptome
© 2018 The Authors
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2018-04-01
2024-04-23
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Transcriptome analysis of a Pseudomonas aeruginosasn-glycerol-3-phosphate dehydrogenase mutant reveals a disruption in bioenergetics
Microbiology 164, 551 (2018); https://doi.org/10.1099/mic.0.000646
/content/journal/micro/10.1099/mic.0.000646
/content/journal/micro/10.1099/mic.0.000646
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