RT Journal Article SR Electronic(1) A1 Shuman, Jon A1 Giles, Tyler Xavier A1 Carroll, Leslie A1 Tabata, Kenji A1 Powers, Austin A1 Suh, Sang-Jin A1 Silo-Suh, LauraYR 2018 T1 Transcriptome analysis of a Pseudomonas aeruginosasn-glycerol-3-phosphate dehydrogenase mutant reveals a disruption in bioenergetics JF Microbiology, VO 164 IS 4 SP 551 OP 562 DO https://doi.org/10.1099/mic.0.000646 PB Microbiology Society, SN 1465-2080, AB Pseudomonas aeruginosa causes acute and chronic human infections and is the major cause of morbidity and mortality in cystic fibrosis (CF) patients. We previously determined that the sn-glycerol-3-phosphate dehydrogenase encoded by glpD plays a larger role in P. aeruginosa physiology beyond its role in glycerol metabolism. To better understand the effect of a glpD mutation on P. aeruginosa physiology we compared the transcriptomes of P. aeruginosa strain PAO1 and the PAO1ΔglpD mutant using RNA-seq analysis. We determined that a null mutation of glpD significantly altered amino acid metabolism in P. aeruginosa and affected the production of intermediates that are channelled into the tricarboxylic acid cycle. Moreover, the loss of glpD induced a general stress response mediated by RpoS in P. aeruginosa. Several other phenotypes observed for the P. aeruginosa glpD mutant include increased persister cell formation, reduced extracellular ATP accumulation and increased heat output. Taken together, these findings implicate sn-glycerol-3-phosphate dehydrogenase as a key player in energy metabolism in P. aeruginosa., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000646