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Volume 164, Issue 5

EssC is a specificity determinant for type VII secretion Open Access

Abstract

The type VII protein secretion system (T7SS) is found in actinobacteria and firmicutes, and plays important roles in virulence and interbacterial competition. A membrane-bound ATPase protein, EssC in , lies at the heart of the secretion machinery. The EssC protein from strains can be grouped into four variants (EssC1–EssC4) that display sequence variability in the C-terminal region. Here we show that the EssC2, EssC3 and EssC4 variants can be produced in a strain deleted for , and that they are able to mediate secretion of EsxA, an essential component of the secretion apparatus. They are, however, unable to support secretion of the substrate protein EsxC, which is only encoded in -specific strains. This finding indicates that EssC is a specificity determinant for T7 protein secretion. Our results support a model in which the C-terminal domain of EssC interacts with substrate proteins, whereas EsxA interacts elsewhere.

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  • Accepted:
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Keyword(s): protein secretion , Staphylococcus aureus , substrate recognition and Type VII secretion
© 2018 The Authors
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2018-05-01
2024-04-20
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EssC is a specificity determinant for Staphylococcus aureus type VII secretion
Microbiology 164, 816 (2018); https://doi.org/10.1099/mic.0.000650
/content/journal/micro/10.1099/mic.0.000650
/content/journal/micro/10.1099/mic.0.000650
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