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Volume 164, Issue 5

Chitin-induced T6SS in is dependent on ChiS activation Free

Abstract

regularly colonizes the chitinous exoskeleton of crustacean shells in the aquatic region. The type 6 secretion system (T6SS) in is an interbacterial killing device. This system is thought to provide a competitive advantage to in a polymicrobial community of the aquatic region under nutrient-poor conditions. chitin sensing is known to be initiated by the activation of a two-component sensor histidine kinase ChiS in the presence of GlcNAc (N,N′-diacetylchitobiose) residues generated by the action of chitinases on chitin. It is known that T6SS in is generally induced by chitin. However, the effect of ChiS activation on T6SS is unknown. Here, we found that ChiS inactivation resulted in impaired bacterial killing and reduced expression of T6SS genes. Active ChiS positively affected T6SS-mediated natural transformation in . ChiS depletion or inactivation also resulted in reduced colonization on insoluble chitin surfaces. Therefore, we have shown that colonization on chitinous surfaces activates ChiS, which promotes T6SS-dependent bacterial killing and horizontal gene transfer. We also highlight the importance of chitinases in T6SS upregulation.

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  • Accepted:
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Keyword(s): ChiS activation , chitin , chitinases , T6SS and Vibrio cholerae
© 2018 The Authors
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2018-05-01
2024-04-19
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Chitin-induced T6SS in Vibrio cholerae is dependent on ChiS activation
Microbiology 164, 751 (2018); https://doi.org/10.1099/mic.0.000656
/content/journal/micro/10.1099/mic.0.000656
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