RT Journal Article SR Electronic(1) A1 Ludwig, Petra A1 Sévin, Daniel C. A1 Busche, Tobias A1 Kalinowski, Jörn A1 Bourdeaux, Florian A1 Grininger, Martin A1 Mack, MatthiasYR 2018 T1 Characterization of the small flavin-binding dodecin in the roseoflavin producer Streptomyces davawensis JF Microbiology, VO 164 IS 6 SP 908 OP 919 DO https://doi.org/10.1099/mic.0.000662 PB Microbiology Society, SN 1465-2080, AB Genes encoding dodecin proteins are present in almost 20 % of archaeal and in more than 50 % of bacterial genomes. Archaeal dodecins bind riboflavin (vitamin B2), are thought to play a role in flavin homeostasis and possibly also help to protect cells from radical or oxygenic stress. Bacterial dodecins were found to bind riboflavin-5′-phosphate (also called flavin mononucleotide or FMN) and coenzyme A, but their physiological function remained unknown. In this study, we set out to investigate the relevance of dodecins for flavin metabolism and oxidative stress management in the phylogenetically related bacteria Streptomyces coelicolor and Streptomyces davawensis. Additionally, we explored the role of dodecins with regard to resistance against the antibiotic roseoflavin, a riboflavin analogue produced by S. davawensis. Our results show that the dodecin of S. davawensis predominantly binds FMN and is neither involved in roseoflavin biosynthesis nor in roseoflavin resistance. In contrast to S. davawensis, growth of S. coelicolor was not reduced in the presence of plumbagin, a compound, which induces oxidative stress. Plumbagin treatment stimulated expression of the dodecin gene in S. davawensis but not in S. coelicolor. Deletion of the dodecin gene in S. davawensis generated a recombinant strain which, in contrast to the wild-type, was fully resistant to plumbagin. Subsequent metabolome analyses revealed that the S. davawensis dodecin deletion strain exhibited a very different stress response when compared to the wild-type indicating that dodecins broadly affect cellular physiology., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000662