RT Journal Article SR Electronic(1) A1 Godlee, Camilla A1 Cerny, Ondrej A1 Durkin, Charlotte H. A1 Holden, David W.YR 2019 T1 SrcA is a chaperone for the Salmonella SPI-2 type three secretion system effector SteD JF Microbiology, VO 165 IS 1 SP 15 OP 25 DO https://doi.org/10.1099/mic.0.000732 PB Microbiology Society, SN 1465-2080, AB Effector proteins of type three secretion systems (T3SS) often require cytosolic chaperones for their stabilization, to interact with the secretion machinery and to enable effector delivery into host cells. We found that deletion of srcA, previously shown to encode a chaperone for the Salmonella pathogenicity island 2 (SPI-2) T3SS effectors SseL and PipB2, prevented the reduction of mature Major Histocompatibility Complex class II (mMHCII) from the surface of antigen-presenting cells during Salmonella infection. This activity was shown previously to be caused by the SPI-2 T3SS effector SteD. Since srcA and steD are located in the same operon on the Salmonella chromosome, this suggested that the srcA phenotype might be due to an indirect effect on SteD. We found that SrcA is not translocated by the SPI-2 T3SS but interacts directly and forms a stable complex with SteD in bacteria with a 2 : 1 stoichiometry. We found that SrcA was not required for SPI-2 T3SS-dependent, neutral pH-induced secretion of either SseL or PipB2 but was essential for secretion of SteD. SrcA therefore functions as a chaperone for SteD, explaining its requirement for the reduction in surface levels of mMHCII., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.000732