Multiplex PCR to detect pAmpC β-lactamases among enterobacteriaceae at a tertiary care laboratory in Mumbai, India
Kazi, Mubin and Ajbani, Kanchan and Tornheim, Jeffrey A. and Shetty, Anjali and Rodrigues, Camilla,, 165, 246-250 (2019),
doi = https://doi.org/10.1099/mic.0.000748,
publicationName = Microbiology Society,
issn = 1350-0872,
abstract= Drug-resistance due to AmpC β-lactamases represents a growing problem worldwide. In this study, a previously collected sample of 108 cefoxitin-resistant clinical isolates was assessed for AmpC β-lactamase production through routine phenotypic testing and double-disc cefoxitin/cloxcallin (DD-CC), cefoxitin/phenylboronic acid (CDT-PBA) and AmpC disc tests. The same isolates were characterized by a novel multiplex polymerase chain reaction molecular assay to detect the presence of blaACT , blaDHA , blaCIT , blaFOX , blaMIR and blaMOX. By phenotypic analysis, 56%, 55% and 48 % were detected as being AmpC β-lactamase producers by the CDT-PBA, DD-CC and AmpC disc tests, respectively. By molecular analysis, 57  % were determined to be AmpC β-lactamase producers, including 34 % blaFOX , 8 % blaCIT and 1.6 % blaDHA as mono-AmpC producers. The production of multiple AmpC molecular types was common, including 30 % with both blaCIT+FOX  and 1.6 % each of blaCIT+DHA , blaACT+MIR , blaACT+FOX , blaACT+DHA and blaMIR+FOX . Molecular characterization of AmpC would help detect the prevalence of AmpC β-lactamase producers, facilitate proper patient management and implement infection control practices.,
language=,
type=