Sharpea azabuensis: a ruminal bacterium that produces trans-11 intermediates from linoleic and linolenic acid Dewanckele, Lore and Vlaeminck, Bruno and Fievez, Veerle,, 165, 772-778 (2019), doi = https://doi.org/10.1099/mic.0.000811, publicationName = Microbiology Society, issn = 1350-0872, abstract= To investigate the metabolism of 18:2n-6 and 18:3n-3 by pure cultures of Sharpea azabuensis , two different strains (RL 1 and ST18) were each incubated in the presence of 40 µg ml-1 18:2n-6 or 18:3n-3. Pure cultures of Butyrivibrio fibrisolvens D1 and Butyrivibrio proteoclasticus P18 were included as control treatments. Similar to the metabolism of B. fibrisolvens , both S. azabuensis strains converted 18:2n-6 or 18:3n-3 to cis-9, trans-11 CLA or cis-9, trans-11, cis-15 CLnA, after which it was further reduced to trans-11 18:1 or trans-11, cis-15 18:2, respectively. B. proteoclasticus additionally reduced trans-11 18:1 to 18:0. Trans-11, cis-15 18:2 was also further metabolized by B. proteoclasticus , although trans-11 18:1 did not accumulate, and only minor amounts of 18:0 were formed. The time frame of 18:2n-6 and 18:3n-3 biohydrogenation by S. azabuensis was comparable with B. fibrisolvens , indicating that S. azabuensis and B. fibrisolvens might be alternative biohydrogenators of 18:2n-6 and 18:3n-3 in the rumen., language=, type=