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Volume 155, Issue 11

Identification and distribution of genetic markers in three closely related taxa of the cluster: refining the relative position and boundaries of the sp. bovine group 7 taxon () Free

Abstract

Mycoplasmas belonging to the phylogenetic cluster are all important ruminant pathogens that are genetically closely related but differ in terms of severity and prevalence of the associated diseases. They are distributed among six taxa, the description of which has recently been amended. In the present study, DNA fragments that diverge between the type strains of three taxa were enriched using suppression subtractive hybridization. Of the three taxa, two were representative of the well-established species and , while the third one, sp. bovine group 7 (7), has only recently been proposed as a separate species, . Specific DNA fragments were further characterized by sequencing and used as markers to assess the genetic diversity within and between taxa. The data indicate that the selected markers are unequally distributed within their own taxon but also across taxa. The patterns observed suggest the occurrence of a genetic continuum of strains within the cluster that may compromise the boundaries between taxa and, in turn, diagnosis outcomes. For 7, the overall nature and distribution of the markers indicate a rather homogeneous group that is distinct from the and species and might be considered as a genomic chimera between these two species.

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Keyword(s): CK, California Kid , ICE, integrative conjugative element , Mcc, Mycoplasma capricolum subsp. capricolum , Mccp, Mycoplasma capricolum subsp. capripneumoniae , Mgb7, Mycoplasma sp. bovine group 7 , Mmc, Mycoplasma mycoides capri , MmmLC, Mycoplasma mycoides subsp. mycoides biotype Large Colony , MmmSC, Mycoplasma mycoides subsp. mycoides biotype Small Colony , SSH, suppression subtractive hybridization , wgs, whole genome sequence shotgun and YG, Y-Goat
SGM
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2009-11-01
2024-04-19
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References

  1. Abu-Groun E. A. M., Taylor R. R., Varsani H., Wadher B. J., Leach R. H., Miles R. J. 1994; Biochemical diversity within the ‘ Mycoplasma mycoides cluster’. Microbiology 140:2033–2042
     [Google Scholar]
  2. Al-Aubaidi J.-M., Fabricant J. 1971; Characterization and classification of bovine mycoplasma. Cornell Vet 61:490–518
     [Google Scholar]
  3. Alexander P. G., Slee K. J., McOrist S., Ireland L., Coloe P. J. 1985; Mastitis in cows and polyarthritis and pneumonia caused by Mycoplasma species bovine group 7. Aust Vet J 62:135–136
     [Google Scholar]
  4. Askaa G., Ernø H., Ojo M. O. 1978; Bovine mycoplasmas: classification of groups related to Mycoplasma mycoides . Acta Vet Scand 19:166–178
     [Google Scholar]
  5. Ayling R. D., Bashiruddin S. E., Nicholas R. A. 2004; Mycoplasma species and related organisms isolated from ruminants in Britain between 1990 and 2000. Vet Rec 155:413–416
     [Google Scholar]
  6. Bergonier D., Berthelot X., Poumarat F. 1997; Contagious agalactia of small ruminants: current knowledge concerning epidemiology, diagnosis and control. Rev Sci Tech 16:848–873
     [Google Scholar]
  7. Bower K., Djordjevic S. P., Andronicos N. M., Ranson M. 2003; Cell surface antigens of Mycoplasma species bovine group 7 bind to and activate plasminogen. Infect Immun 71:4823–4827
     [Google Scholar]
  8. Brown D. R., Bradbury J. M. 2008; International commitee on systematics of procaryotes – subcommittee on the taxonomy of mollicutes. Minutes of the meetings, 6 and 11 July 2008, Tianjin, PR China. Int J Syst Evol Microbiol 58:2987–2990
     [Google Scholar]
  9. Burrus V., Waldor M. K. 2004; Shaping bacterial genomes with integrative and conjugative elements. Res Microbiol 155:376–386
     [Google Scholar]
  10. Christiansen C., Ernø H. 1982; Classification of the F38 group of caprine mycoplasma strains by DNA hybridization. J Gen Microbiol 128:2523–2526
     [Google Scholar]
  11. Connole M. D., Laws L., Hart R. K. 1967; Mastitis in cattle caused by a Mycoplasma sp. Aust Vet J 43:157–162
     [Google Scholar]
  12. Costas M., Leach R. H., Mitchelmore D. L. 1987; Numerical analysis of PAGE protein patterns and the taxonomic relationships within the ‘ Mycoplasma mycoides cluster’. J Gen Microbiol 133:3319–3329
     [Google Scholar]
  13. Cottew G. S., Breard A., DaMassa A. J., Ernø H., Leach R. H., Lefèvre P. C., Rodwell A. W., Smith G. R. 1987; Taxonomy of the Mycoplasma mycoides cluster. Isr J Med Sci 23:632–635
     [Google Scholar]
  14. DaMassa A. J., Wakenell P. S., Brooks D. L. 1992; Mycoplasmas of goats and sheep. J Vet Diagn Invest 4:101–113
     [Google Scholar]
  15. Djordjevic S. R., Forbes W. A., Forbes-Faulkner J., Kuhnert P., Hum S., Hornitzky M. A., Vilei E. M., Frey J. 2001; Genetic diversity among Mycoplasma species bovine group 7: clonal isolates from an outbreak of mastitis, and abortion in dairy cattle. Electrophoresis 22:3551–3561
     [Google Scholar]
  16. Djordjevic S. P., Vilei E. M., Frey J. 2003; Characterization of a chromosomal region of Mycoplasma sp. bovine group 7 strain PG50 encoding a glycerol transport locus (GtsABC. Microbiology 149:195–204
     [Google Scholar]
  17. Frey J. 2002; Mycoplasmas of animals. In Molecular Biology and Pathogenicity of Mycoplasmas pp 73–90 Edited by Razin S., Herrmann R. New York: Kluwer Academic/Plenum Publishers;
     [Google Scholar]
  18. Frey J., Cheng X., Kuhnert P., Nicolet J. 1995; Identification and characterization of IS 1296 in Mycoplasma mycoides subsp. mycoides SC and presence in related mycoplasmas. Gene 160:95–100
     [Google Scholar]
  19. Frey J., Cheng X., Monnerat M. P., Abdo E. M., Krawinkler M., Bolske G., Nicolet J. 1998; Genetic and serological analysis of the immunogenic 67-kDa lipoprotein of Mycoplasma sp. bovine group 7. Res Microbiol 149:55–64
     [Google Scholar]
  20. Harasawa R., Hotzel H., Sachse K. 2000; Comparison of the 16S–23S rRNA intergenic spacer regions among strains of the Mycoplasma mycoides cluster, and reassessment of the taxonomic position of Mycoplasma sp. bovine group 7. Int J Syst Evol Microbiol 50:1325–1329
     [Google Scholar]
  21. Huang X., Madan A. 1999; CAP3: A DNA sequence assembly program. Genome Res 9:868–877
     [Google Scholar]
  22. Hum S., Kessell A., Djordjevic S., Rheinberger R., Hornitzky M., Forbes W., Gonsalves J. 2000; Mastitis, polyarthritis and abortion caused by Mycoplasma species bovine group 7 in dairy cattle. Aust Vet J 78:744–750
     [Google Scholar]
  23. Kusiluka L. J. M., Ojeniyi B., Friis N. F., Kokotovic B., Ahrens P. 2001; Molecular analysis of field strains of Mycoplasma capricolum subspecies capripneumoniae and Mycoplasma mycoides subspecies mycoides, small colony type isolated from goats in Tanzania. Vet Microbiol 82:27–37
     [Google Scholar]
  24. Lartigue C., Glass J. I., Alperovich N., Pieper R., Parmar P. P., Hutchison C. A. III, Smith H. O., Venter J. C. 2007; Genome transplantation in bacteria: changing one species to another. Science 317:632–638
     [Google Scholar]
  25. Le Grand D., Saras E., Blond D., Solsona M., Poumarat F. 2004; Assessment of PCR for routine identification of species of the Mycoplasma mycoides cluster in ruminants. Vet Res 35:635–649
     [Google Scholar]
  26. Maigre L., Citti C., Marenda M., Poumarat F., Tardy F. 2008; Suppression-subtractive hybridization as a strategy to identify taxon-specific sequences within the Mycoplasma mycoides Cluster: design and validation of a M. capricolum subsp. capricolum specific PCR assay. J Clin Microbiol 46:1307–1316
     [Google Scholar]
  27. Manso-Silvan L., Perrier X., Thiaucourt F. 2007; Phylogeny of the Mycoplasma mycoides cluster based on analysis of five conserved protein-coding sequences and possible implications for the taxonomy of the group. Int J Syst Evol Microbiol 57:2247–2258
     [Google Scholar]
  28. Manso-Silvan L., Vilei E. M., Sachse K., Djordjevic S. P., Thiaucourt F., Frey J. 2009; Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoidessubsp. capri . Int J Syst Evol Microbiol 59:1353–1358
     [Google Scholar]
  29. Monnerat M. P., Thiaucourt F., Poveda J. B., Nicolet J., Frey J. 1999; Genetic and serological analysis of lipoprotein LppA in Mycoplasma mycoides subsp. mycoides LC and Mycoplasma mycoides subsp capri . Clin Diagn Lab Immunol 6:224–230
     [Google Scholar]
  30. Olsson B., Bolske G., Bergstrom K., Johansson K. E. 1990; Analysis of caprine mycoplasmas and mycoplasma infections in goats using two-dimensional electrophoresis and immunoblotting. Electrophoresis 11:861–869
     [Google Scholar]
  31. Pettersson B., Leitner T., Ronaghi M., Bölske G., Uhlèn M., Johansson K. E. 1996; Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons. J Bacteriol 178:4131–4142
     [Google Scholar]
  32. Pilo P., Vilei E. M., Peterhans E., Bonvin-Klotz L., Stoffel M. H., Dobbelaere D., Frey J. 2005; A metabolic enzyme as a primary virulence factor of Mycoplasma mycoides subsp. mycoides small colony. J Bacteriol 187:6824–6831
     [Google Scholar]
  33. Poumarat F., Longchambon D., Martel J.-L. 1992; Application of dot immunobinding on membrane filtration (MF dot) to the study of relationships within “ M. mycoides cluster” and within “glucose and arginine-negative cluster” of ruminant mycoplasmas. Vet Microbiol 32:375–390
     [Google Scholar]
  34. Poumarat F., Le Grand D., Mercier P., Tardy F., Gaurivaud P., Calavas D. 2006; VIGIMYC, le réseau français d'épidémio-surveillance des mycoplasmoses des ruminants, bilan 2003–2005. Le Nouveau Praticien Vétérinaire 3:22–26
     [Google Scholar]
  35. Razin S., Yogev D., Naot Y. 1998; Molecular biology and pathogenicity of mycoplasmas. Microbiol Mol Biol Rev 62:1094–1156
     [Google Scholar]
  36. R-Development_Core_Team 2009 R: A Language and Environment for Statistical Computing R Foundation for Statistical Computing;
     [Google Scholar]
  37. Salih M. M., Erno H., Simonsen V. 1983; Electrophoretic analysis of isoenzymes of mycoplasma species. Acta Vet Scand 24:14–33
     [Google Scholar]
  38. Shiel M. J., Coloe P. J., Worotniuk B., Burgess G. W. 1982; Polyarthritis in a calf associated with group 7 mycoplasma infection. Aust Vet J 59:192–193
     [Google Scholar]
  39. Simmons G. C., Johnston L. A. Y. 1963; Arthritis in calves caused by Mycoplasma sp. Aust Vet J 39:11–14
     [Google Scholar]
  40. Sirand-Pugnet P., Lartigue C., Marenda M., Jacob D., Barré A., Barbe V., Schenowitz C., Mangenot S., Couloux A. other authors 2007; Being pathogenic, plastic, and sexual while living with a nearly minimal bacterial genome. PLoS Genet 3:e75
     [Google Scholar]
  41. Thiaucourt F., Bölske G. 1996; Contagious caprine pleuropneumonia and other pulmonary mycoplasmoses of sheep and goats. Rev Sci Tech 15:1397–1414
     [Google Scholar]
  42. Thiaucourt F., Lorenzon S., David A., Breard A. 2000; Phylogeny of the Mycoplasma mycoides cluster as shown by sequencing of a putative membrane protein gene. Vet Microbiol 72:251–268
     [Google Scholar]
  43. Thomas A., Ball H., Dizier I., Trolin A., Bell C., Mainil J., Linden A. 2002; Isolation of Mycoplasma species from the lower respiratory tract of healthy cattle and cattle with respiratory disease in Belgium. Vet Rec 151:472–476
     [Google Scholar]
  44. Vilei E. M., Frey J. 2001; Genetic and biochemical characterization of glycerol uptake in Mycoplasma mycoides subsp. mycoides SC: its impact on H2O2 production and virulence. Clin Diagn Lab Immunol 8:85–92
     [Google Scholar]
  45. Vilei E. M., Korczak B. M., Frey J. 2006; Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be grouped into a single subspecies. Vet Res 37:779–790
     [Google Scholar]
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Identification and distribution of genetic markers in three closely related taxa of the Mycoplasma mycoides cluster: refining the relative position and boundaries of the Mycoplasma sp. bovine group 7 taxon (Mycoplasma leachii)
Microbiology 155, 3775 (2009); https://doi.org/10.1099/mic.0.030528-0
/content/journal/micro/10.1099/mic.0.030528-0
/content/journal/micro/10.1099/mic.0.030528-0
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