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Volume 157, Issue 11

Role of sialidase in glycoprotein utilization by Open Access

Abstract

The major bacterial pathogens associated with periodontitis include . We previously discovered that sialic acid stimulates biofilm growth of , and that sialidase activity is key to utilization of sialoconjugate sugars and is involved in host–pathogen interactions . The aim of this work was to assess the influence of the NanH sialidase on initial biofilm adhesion and growth in experiments where the only source of sialic acid was sialoglycoproteins or human oral secretions. After showing that can utilize sialoglycoproteins for biofilm growth, we showed that growth and initial adhesion with sialylated mucin and fetuin were inhibited two- to threefold by the sialidase inhibitor oseltamivir. A similar reduction (three- to fourfold) was observed with a mutant compared with the wild-type. Importantly, these data were replicated using clinically relevant serum and saliva samples as substrates. In addition, the ability of the mutant to form biofilms on glycoprotein-coated surfaces could be restored by the addition of purified NanH, which we show is able to cleave sialic acid from the model glycoprotein fetuin and, much less efficiently, 9--acetylated bovine submaxillary mucin. These data show for the first time that glycoprotein-associated sialic acid is likely to be a key nutrient source for when growing in a biofilm, and suggest that sialidase inhibitors might be useful adjuncts in periodontal therapy.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Funding
This study was supported by the:
  • Royal Society
  • Dunhill Medical Trust
  • British Oral and Dental Research Trust
  • University of Sheffield Oral Disease cluster studentship
  • Wellcome Trust Value in People Fellowship
  • US Public Health Grant (Award DE014749)
© 2011 SGM
  • This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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2011-11-01
2024-04-20
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Role of sialidase in glycoprotein utilization by Tannerella forsythia
Microbiology 157, 3195 (2011); https://doi.org/10.1099/mic.0.052498-0
/content/journal/micro/10.1099/mic.0.052498-0
/content/journal/micro/10.1099/mic.0.052498-0
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