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Volume 158, Issue 5

Inner-membrane transporters for the siderophores pyochelin in and enantio-pyochelin in display different enantioselectivities Free

Abstract

Iron uptake and transcriptional regulation by the enantiomeric siderophores pyochelin (Pch) and enantio-pyochelin (EPch) of and , respectively, are stereospecific processes. The iron-loaded forms of Pch (ferriPch) and of EPch (ferriEPch) are recognized stereospecifically (i) at the outer membrane by the siderophore receptors FptA in and FetA in and (ii) in the cytoplasm by the two AraC-type regulators PchR, which are activated by their cognate siderophore. Here, stereospecific siderophore recognition is shown to occur at the inner membrane also. In , translocation of ferriPch across the inner membrane is carried out by the single-subunit siderophore transporter FptX. In contrast, the uptake of ferriEPch into the cytoplasm of was found to involve a classical periplasmic binding protein-dependent ABC transporter (FetCDE), which is encoded by the operon. Expression of a translational fusion was repressed by ferric ions, and activated by the cognate siderophore bound to PchR, thus resembling the analogous regulation of the ferriPch transport operon . The inner-membrane transporters FetCDE and FptX were expressed in combination with either of the two siderophore receptors FetA and FptA in a siderophore-negative mutant deleted for the operon. Growth tests conducted under iron limitation with ferriPch or ferriEPch as the iron source revealed that FptX was able to transport ferriPch as well as ferriEPch, whereas FetCDE specifically transported ferriEPch. Thus, stereospecific siderophore recognition occurs at the inner membrane by the FetCDE transporter.

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2012-05-01
2024-04-23
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Inner-membrane transporters for the siderophores pyochelin in Pseudomonas aeruginosa and enantio-pyochelin in Pseudomonas fluorescens display different enantioselectivities
Microbiology 158, 1317 (2012); https://doi.org/10.1099/mic.0.057430-0
/content/journal/micro/10.1099/mic.0.057430-0
/content/journal/micro/10.1099/mic.0.057430-0
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