RT Journal Article SR Electronic(1) A1 Lee, Dong Hwan A1 Lim, Jeong-A A1 Lee, Juneok A1 Roh, Eunjung A1 Jung, Kyusuk A1 Choi, Minseon A1 Oh, Changsik A1 Ryu, Sangryeol A1 Yun, Jongchul A1 Heu, SunggiYR 2013 T1 Characterization of genes required for the pathogenicity of Pectobacterium carotovorum subsp. carotovorum Pcc21 in Chinese cabbage JF Microbiology, VO 159 IS Pt_7 SP 1487 OP 1496 DO https://doi.org/10.1099/mic.0.067280-0 PB Microbiology Society, SN 1465-2080, AB Pectobacterium carotovorum subsp. carotovorum is a well-known plant pathogen that causes severe soft rot disease in various crops, resulting in considerable economic loss. To identify pathogenicity-related factors, Chinese cabbage was inoculated with 5314 transposon mutants of P. carotovorum subsp. carotovorum Pcc21 derived using Tn5 transposon mutagenesis. A total of 35 reduced-virulence or avirulent mutants were isolated, and 14 loci were identified. The 14 loci could be functionally grouped into nutrient utilization (pyrD, purH, purD, leuA and serB), production of plant cell-wall-degrading enzymes (PCWDEs) (expI, expR and PCC21_023220), motility (flgA, fliA and flhB), biofilm formation (expI, expR and qseC), susceptibility to antibacterial plant chemicals (tolC) and unknown function (ECA2640). Among the 14 genes identified, qseC, tolC and PCC21_023220 are novel pathogenicity factors of P. carotovorum subsp. carotovorum involved in biofilm formation, phytochemical resistance and PCWDE production, respectively., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.067280-0