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Volume 159, Issue Pt_10

Cell division, one-carbon metabolism and methionine synthesis in a -deficient mutant, and a role for MmuM Free

Abstract

An K-12 mutant deficient in -adenosylmethionine (SAM) synthesis, i.e Δ, but expressing a rickettsial SAM transporter, can grow in glucose minimal medium if provided with both SAM and methionine. It uses the externally provided ()-enantiomer of SAM as methyl donor to produce most but not all of its methionine, by methylation of homocysteine catalysed by homocysteine methyltransferase (MmuM). The Δ cells are also altered in growth and are twice as long as those of the parent strain. When starved of SAM, the mutant makes a small proportion of very long cells suggesting a role of SAM and of methylation in the onset of crosswall formation.

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2013-10-01
2024-04-26
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Cell division, one-carbon metabolism and methionine synthesis in a metK-deficient Escherichia coli mutant, and a role for MmuM
Microbiology 159, 2036 (2013); https://doi.org/10.1099/mic.0.069682-0
/content/journal/micro/10.1099/mic.0.069682-0
/content/journal/micro/10.1099/mic.0.069682-0
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