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Volume 159, Issue Pt_11

A synthetic system for expression of components of a bacterial microcompartment Free

Abstract

In general, prokaryotes are considered to be single-celled organisms that lack internal membrane-bound organelles. However, many bacteria produce proteinaceous microcompartments that serve a similar purpose, i.e. to concentrate specific enzymic reactions together or to shield the wider cytoplasm from toxic metabolic intermediates. In this paper, a synthetic operon encoding the key structural components of a microcompartment was designed based on the genes for the propanediol utilization (Pdu) microcompartment. The genes chosen included , -, -, -, -, - and -, and each was shown to produce protein in an chassis. In parallel, a set of compatible vectors designed to express non-native cargo proteins was also designed and tested. Engineered hexa-His tags allowed isolation of the components of the microcompartments together with co-expressed, untagged, cargo proteins. Finally, an protease accessibility assay suggested that a PduD–GFP fusion could be protected from proteolysis when co-expressed with the synthetic microcompartment operon. This work gives encouragement that it may be possible to harness the genes encoding a non-native microcompartment for future biotechnological applications.

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  • Accepted:
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2013-11-01
2024-04-23
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A synthetic system for expression of components of a bacterial microcompartment
Microbiology 159, 2427 (2013); https://doi.org/10.1099/mic.0.069922-0
/content/journal/micro/10.1099/mic.0.069922-0
/content/journal/micro/10.1099/mic.0.069922-0
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