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Volume 160, Issue 11

Control of zinc homeostasis in via and the zinc uptake genes and Free

Abstract

The zinc uptake regulator (Zur) was shown to negatively regulate the zinc uptake genes , encoding a zinc transport system belonging to the ATP-binding cassette (ABC) transporter family, and , which encodes a periplasmic zinc-binding protein. The expression of and was inducible when cells were grown in medium containing a metal chelator (EDTA), and this induction was shown to be specific for zinc depletion. The expression of was reduced in response to increased zinc in a dose-dependent manner, and had a less pronounced but similar pattern of zinc-regulated expression. The inactivation of led to constitutively high expression of and . In addition, a mutant had an increased total zinc content compared to the WT NTL4 strain, whereas the inactivation of caused a reduction in the total zinc content. The gene is shown to play a dominant role and to be more important than and for survival under zinc deprivation. ZinT can function even when ZnuABC is inactivated. However, mutations in , , or did not affect the virulence of .

  • Received:
  • Accepted:
  • Published Online:
Funding
This study was supported by the:
  • Thailand Research Fund Royal Golden Jubilee Scholarship (Award PHD52K0207)
  • Chulabhorn Research Institute
  • Thailand Research Fund (Award RSA5380004)
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2014-11-01
2024-04-24
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Control of zinc homeostasis in Agrobacterium tumefaciens via zur and the zinc uptake genes znuABC and zinT
Microbiology 160, 2452 (2014); https://doi.org/10.1099/mic.0.082446-0
/content/journal/micro/10.1099/mic.0.082446-0
/content/journal/micro/10.1099/mic.0.082446-0
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