@article{mbs:/content/journal/micro/10.1099/mic.0.2006/001453-0, author = "Yamada, Takashi and Kawasaki, Takeru and Nagata, Shoko and Fujiwara, Akiko and Usami, Shoji and Fujie, Makoto", title = "New bacteriophages that infect the phytopathogen Ralstonia solanacearum", journal= "Microbiology", year = "2007", volume = "153", number = "8", pages = "2630-2639", doi = "https://doi.org/10.1099/mic.0.2006/001453-0", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.2006/001453-0", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Four kinds of bacteriophage (φRSL, φRSA, φRSM and φRSS) were isolated from Ralstonia solanacearum, a soil-borne Gram-negative bacterium that is the causative agent of bacterial wilt in many important crops. The Myovirus-type phages φRSL1 and φRSA1 contained dsDNA genomes of 240 kbp and 39 kbp, respectively. These phages have relatively wide host ranges and gave large clear plaques with various host strains; especially φRSA1 was able to infect all 15 R. solanacearum strains of different races or different biovars tested in this study. Three host strains contained φRSA1-related sequences in their genomic DNAs, suggesting a lysogenic cycle of φRSA1. Two phages, φRSM1 and φRSS1, were characterized as Ff-type phages (Inovirus) based on their particle morphology, genomic ssDNA and infection cycle. However, despite their similar fibrous morphology, their genome size (9.0 kb for φRSM1 and 6.6 kb for φRSS1) and genome sequence were different. Strains of R. solanacearum that were sensitive to φRSM1 were resistant to φRSS1 and vice versa. Several R. solanacearum strains contained φRSM1-related sequences and at least one strain produced φRSM1 particles, indicating the lysogenic state of this phage. These phages may be useful as a tool not only for molecular biological studies of R. solanacearum pathogenicity but also for specific and efficient detection (φRSM1 and φRSS1) and control of harmful pathogens (φRSL and φRSA) in cropping ecosystems as well as growing crops.", }