1887

Abstract

Invasin, the major adhesion and invasion factor of , is encoded by the gene, which is regulated by growth phase and in response to a variety of environmental conditions such as temperature, pH and osmolarity. So far, three proteins, RovA, H-NS and YmoA, have been identified as factors regulating the expression of the gene in enteropathogenic Here, data from  : :  chromosomal gene fusion studies are presented indicating that OmpR, the response regulator of the EnvZ/OmpR two-component system, acts to negatively regulate expression at the transcriptional level at 25 °C, and that high osmolarity enhances the inhibitory effect of this protein. In a strain lacking OmpR the expression of at 25 °C was increased sixfold, but at 37 °C, a temperature known to repress expression, this effect was not observed, suggesting that temperature regulation of is OmpR-independent. Furthermore, the expression of in the background was no longer responsive to increased osmolarity. Complementation with the active allele restored wild-type expression in the mutant. analysis of the O : 9 promoter sequence revealed the presence of an OmpR consensus binding site located in the −15 to −33 region. OmpR was able to specifically bind to a fragment of the promoter containing this putative binding site in electrophoretic mobility shift assays. Thus, OmpR seems to be a repressor of in

Loading

Article metrics loading...

/content/journal/micro/10.1099/mic.0.2006/003202-0
2007-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/153/8/2416.html?itemId=/content/journal/micro/10.1099/mic.0.2006/003202-0&mimeType=html&fmt=ahah

References

  1. Baumler A. J., Tsolis R. M., Van der Velden A. W. M., Stojiljkovic I., Anic S., Heffron A. 1996; Identification of a new iron regulated locus of Salmonella typhi . Gene 183:207–213
    [Google Scholar]
  2. Branlant G., Branlant C. 1985; Nucleotide sequence of the Escherichia coli gap gene. Different evolutionary behavior of the NAD+- binding domain and of the catalytic domain of d-glyceraldehyde-3-phosphate dehydrogenase. Eur J Biochem 150:61–66
    [Google Scholar]
  3. Brubaker R. R. 1991; Factors promoting acute and chronic diseases caused by yersiniae. Clin Microbiol Rev 4:309–324
    [Google Scholar]
  4. Brzostek K., Raczkowska A. 2003; The osmotic regulator OmpR is involved in the response of Yersinia enterocolitica O : 9 to environmental stresses and survival within macrophages. FEMS Microbiol Lett 228:265–271
    [Google Scholar]
  5. Brzostek K., Raczkowska A. 2007; The YompC protein of Yersinia enterocolitica : molecular and physiological characterization. Folia Microbiol 52:73–80
    [Google Scholar]
  6. Cornelis G. R., Boland A., Boyd A. P., Geuijen C., Iriarte M., Neyt C., Sory M., Stainier I. 1998; The virulence plasmid of Yersinia , an antihost genome. Microbiol Mol Biol Rev 62:1315–1352
    [Google Scholar]
  7. Dorrell N., Li S. R., Everest P. H., Dougan G., Wren B. W. 1998; Construction and characterisation of a Yersinia enterocolitica O : 8 ompR mutant. FEMS Microbiol Lett 165:145–151
    [Google Scholar]
  8. Egger L. A., Park H., Inouye M. 1997; Signal transduction via the histidyl–aspartyl phosphorelay. Genes Cells 2:167–184
    [Google Scholar]
  9. Ellison D. W., Miller V. L. 2006a; Regulation of virulence by members of the MarR/SlyA family. Curr Opin Microbiol 9:153–159
    [Google Scholar]
  10. Ellison D. W., Miller V. L. 2006b; H-NS represses inv transcription in Yersinia enterocolitica through competition with RovA and interaction with YmoA. J Bacteriol 188:5101–5112
    [Google Scholar]
  11. Ellison D. W., Young B., Nelson K., Miller V. L. 2003; YmoA negatively regulates expression of invasin from Yersinia enterocolitica . J Bacteriol 185:7153–7159
    [Google Scholar]
  12. Ellison D. W., Lawrenz M. B., Miller V. L. 2004; Invasin and beyond: regulation of Yersinia virulence by RovA. Trends Microbiol 12:296–300
    [Google Scholar]
  13. Figurski D. H., Helsinki D. R. 1979; Replication of an origin containing derivative of plasmid RK2 dependent on a plasmid function provided in trans . Proc Natl Acad Sci U S A 76:1648–1652
    [Google Scholar]
  14. Heroven A. K., Nagel G., Tran H. J., Parr S., Dersch P. 2004; RovA is autoregulated and antagonizes H-NS-mediated silencing of invasin and rovA expression in Yersinia pseudotuberculosis . Mol Microbiol 53:871–888
    [Google Scholar]
  15. Higashitani A., Nishimura Y., Hara H., Aiba H., Mizuno T., Horiuchi K. 1993; Osmoregulation of the fatty acid receptor gene fadL in Escherichia coli . Mol Gen Genet 240:339–347
    [Google Scholar]
  16. Iriarte M., Vanooteghem J. C., Delor I., Diaz R., Knutton S., Cornelis G. R. 1993; The Myf fibrillae of Yersinia enterocolitica . Mol Microbiol 9:507–520
    [Google Scholar]
  17. Isberg R. R., Van Nhieu G. T. 1995; The mechanism of phagocytic uptake promoted by invasin-integrin interaction. Trends Cell Biol 5:120–124
    [Google Scholar]
  18. Jubelin G., Vianney A., Beloin C., Ghigo J. M., Lazzaroni J. C., Lejeune P., Dorel C. 2005; CpxR/OmpR interplay regulates curli gene expression in response to osmolarity in Escherichia coli . J Bacteriol 187:2038–2049
    [Google Scholar]
  19. Kovach M. E., Elzer P. H., Hill D. S., Robertson G. T., Farris M. A., Roop R. M. II, Peterson K. M. 1995; Four new derivatives of the broad-host-range cloning vector pBBR1MCS, carrying different antibiotic-resistance cassettes. Gene 166:175–176
    [Google Scholar]
  20. Lan C. Y., Igo M. M. 1998; Differential expression of the OmpF and OmpC porin proteins in Escherichia coli K-12 depends upon the level of active OmpR. J Bacteriol 180:171–174
    [Google Scholar]
  21. Lee A. K., Detweiler C. S., Falkow S. 2000; OmpR regulates the two-component system SsrA–SsrB in S almonella pathogenicity island 2. J Bacteriol 182:771–781
    [Google Scholar]
  22. McCleary W. R., Stock J. B. 1994; Acetyl phosphate and the activation of two-component response regulators. J Biol Chem 269:31567–31572
    [Google Scholar]
  23. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  24. Miller V. L., Farmer J. J., Hill W. E., Falkow S. 1989; The ail locus is found uniquely in Yersinia enterocolitica serotypes commonly associated with disease. Infect Immun 57:121–131
    [Google Scholar]
  25. Nagel G., Lahrz A., Dersch P. 2001; Environmental control of invasin expression in Yersinia pseudotuberculosis is mediated by regulation of RovA, a transcriptional activator of the Sly/Hor family. Mol Microbiol 41:1249–1269
    [Google Scholar]
  26. Pepe J. C., Miller V. L. 1993; Yersinia enterocolitica invasin: a primary role in the initiation of infection. Proc Natl Acad Sci U S A 90:6473–6477
    [Google Scholar]
  27. Pepe J. C., Badger J. L., Miller V. L. 1994; Growth phase and low pH affect the thermal regulation of the Yersinia enterocolitica inv gene. Mol Microbiol 11:123–135
    [Google Scholar]
  28. Revell P. A., Miller V. L. 2000; A chromosomally encoded regulator is required for expression of the Yersinia enterocolitica inv gene and for virulence. Mol Microbiol 35:677–685
    [Google Scholar]
  29. Russo F. D., Silhavy T. J. 1991; EnvZ controls the concentration of phosphoryled OmpR to mediate osmoregulation of the porin genes. J Mol Biol 222:567–580
    [Google Scholar]
  30. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd ed. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  31. Schulze-Koops H., Burkhardt H., Heesemann J., von der Mark K., Emmrich F. 1992; Plasmid-encoded outer membrane protein YadA mediates specific binding of enteropathogenic yersiniae to various types of collagen. Infect Immun 60:2153–2159
    [Google Scholar]
  32. Shin S., Park C. 1995; Modulation of flagellar expression in Escherichia coli by acetyl phosphate and the osmoregulator OmpR. J Bacteriol 177:4696–4702
    [Google Scholar]
  33. Simon R., Priefer U., Puhler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Biotechnology 1:784–791
    [Google Scholar]
  34. Skurnik M., El Tahir Y., Saarinen M., Jalkanen S., Toivanen P. 1994; YadA mediates specific binding of enteropathogenic Yersinia enterocolitica to human intestinal submucosa. Infect Immun 62:1252–1261
    [Google Scholar]
  35. Tran H. J., Heroven A. K., Winkler L., Spreter T., Beatrix B., Dersch P. 2005; Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation. J Biol Chem 280:42423–42432
    [Google Scholar]
  36. Yamamoto K., Nagura R., Tanabe H., Fujita N., Ishihama A., Utsumi R. 2000; Negative regulation of the bolA1p of Escherichia coli K-12 by the transcription factor OmpR for osmolarity response genes. FEMS Microbiol Lett 186:257–262
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/mic.0.2006/003202-0
Loading
/content/journal/micro/10.1099/mic.0.2006/003202-0
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error