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Volume 154, Issue 5

A chromosome map of the Flavescence dorée phytoplasma Free

Abstract

The Flavescence dorée phytoplasma (FD-P), a non-cultivable, plant-pathogenic bacterium of the class , is the causal agent of a quarantine disease affecting vineyards of southern Europe, mainly in southern France and northern Italy. To investigate FD-P diversity and phytoplasma genetic determinants governing the FD-P life cycle, a genome project has been initiated. A physical map of the chromosome of FD-P strain FD92, purified from infected broad beans, was constructed by performing restriction digests of the chromosome and resolving the fragments by PFGE. Single and double digestions of the chromosome with the enzymes I, HII, I and I were performed and used to map 13 restriction sites on the FD-P chromosome. The size of the chromosome was calculated to be 671 kbp. Southern blot analyses using cloned phytoplasma probes were carried out to assist in the arrangement of contiguous restriction fragments and to map eight genetic loci, including the two rRNA operons, the , and (FD9) genes, the FD2 marker and two orphan sequences (FDDH29 and FDSH05) isolated through subtractive suppression hybridization.

  • Received:
  • Accepted:
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  • Published Online:
Keyword(s): FD, Flavescence dorée , FD-P, Flavescence dorée phytoplasma and SSH, subtractive suppression hybridization
SGM
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2008-05-01
2024-04-19
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A chromosome map of the Flavescence dorée phytoplasma
Microbiology 154, 1454 (2008); https://doi.org/10.1099/mic.0.2007/013888-0
/content/journal/micro/10.1099/mic.0.2007/013888-0
/content/journal/micro/10.1099/mic.0.2007/013888-0
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