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Abstract

Plasmid SCP2* is a 31 kb, circular, low-copy-number plasmid originally identified in A3(2) as a fertility factor. The plasmid was completely sequenced. The analysis of the 31 317 bp sequence revealed 34 ORFs encoding putative proteins from 31 to 710 aa long, most of them lacking similarity to known proteins. Three functional regions had been identified previously: the replication region, the transfer and spreading region, and the stability region. Three genes were identified in the stability region which contribute to the stability of SCP2 as shown by plasmid stability testing. The first gene, , encodes a new member of the λ integrase family of site-specific recombinases. The two genes downstream of were called and . The gene product, ParA, shows similarity to a family of ATPases involved in plasmid partition. An increase of plasmid stability could be seen only when both genes were present. By deletion analysis, the replication region could be narrowed down to a 1·6 kb region, consisting of a 650 bp non-coding region and two genes, and , encoding proteins of 161 and 131 aa. Only RepI exhibits similarities to DNA binding elements and contains a putative helix–turn–helix motif. The gene that is essential for DNA transfer and pock formation was identified previously. Upstream of , 10 ORFs were found in the same orientation as which might be involved in conjugation and DNA spreading, together with one gene in the opposite orientation with similarities to transcriptional regulators of DNA transfer. Two transposable elements were found on SCP2*. IS belongs to the IS family of insertion sequences. The second element, Tn, shows the highest similarity to the Tn element located in the terminal inverted repeats of the chromosome.

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2003-02-01
2024-03-29
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References

  1. Altenbuchner J., Eichenseer C. 1991; A new system to study DNA amplification in Streptomyces lividans . In Genetics and Product Formation in Streptomyces pp 253–264 Edited by Baumberg S., Krügel H., Noack D. New York: Plenum Press;
    [Google Scholar]
  2. Bentley S. D, Chater K. F, Cardeno-Tarraga A.-M. 39 other authors 2002; Complete genome sequence of the model actinomycete Streptomyces coelicolor A3(2). Nature 417:141–147
    [Google Scholar]
  3. Bibb M. J., Hopwood D. A. 1981; Genetic studies of the fertility plasmid SCP2 and its SCP2* variants in S. coelicolor A3(2). J Gen Microbiol 126:427–442
    [Google Scholar]
  4. Bibb M. J, Freeman R. F., Hopwood D. A. 1977; Physical and genetical characterisation of a second sex factor, SCP2 for Streptomyces coelicolor A3(2). Mol Gen Genet 154:155–166
    [Google Scholar]
  5. Bibb M. J, Schottel J. L., Cohen S. N. 1980; DNA cloning system for interspecifies gene transfer in antibiotic producing Streptomyces . Nature 284:526–531
    [Google Scholar]
  6. Bignell C., Thomas M. 2001; The bacterial ParA–ParB partitioning proteins. J Biotechnol 91:1–34
    [Google Scholar]
  7. Boyer H. W., Rouland-Dussoix D. 1969; A complementation analysis of the restriction and modification of DNA. J Mol Biol 41:459–465
    [Google Scholar]
  8. Brolle D. F, Pape H, Hopwood D. A., Kieser T. 1993; Analysis of the transfer region of the Streptomyces plasmid SCP2*. Mol Microbiol 10:157–170
    [Google Scholar]
  9. Chattoraj D. K. 2000; Control of plasmid DNA replication by iterons: no longer paradoxical. Mol Microbiol 37:467–476
    [Google Scholar]
  10. Chen C. W, Yu T.-W, Chung H.-M., Chou C.-F. 1992; Discovery and characterization of a new transposable element, Tn 4811 , in Streptomyces lividans 66. J Bacteriol 174:7762–7769
    [Google Scholar]
  11. Cole S. T, Brosch R, Parkhill J. 39 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature 393:537–544
    [Google Scholar]
  12. Fischer J, Maier H, Viell P., Altenbuchner J. 1996; The use of an improved transposon mutagenesis system for DNA sequencing leads to the chracterization of a new insertion sequence of Streptomyces lividans 66. Gene 180:81–89
    [Google Scholar]
  13. Gerdes K, Thisted T., Martinussen J. 1990; Mechanism of post-segregational killing by the hok / sok system of plasmid R1: sok antisense RNA regulates formation of a hok mRNA species correlated with killing of plasmid-free cells. Mol Microbiol 4:1807–1818
    [Google Scholar]
  14. Hagege J, Pernodet J. L, Sezonov G, Gerbaud C, Friedmann A., Guerineau M. 1993; Transfer functions of the conjugative integrating element pSAM2 from Streptomyces ambofaciens : characterization of a kil-kor system associated with transfer. J Bacteriol 175:5529–5538
    [Google Scholar]
  15. Hiratsu K, Mochizuki S., Kinashi H. 2000; Cloning and analysis of the replication origin and the telomeres of the large linear plasmid pSLA2-L in Streptomyces rochei . Mol Gen Genet 263:1015–1021
    [Google Scholar]
  16. Hopwood D. A, Kieser T. R, Wright H. M., Bibb M. J. 1983; Plasmids, recombination and chromosome mapping in Streptomyces lividans 66. J Gen Microbiol 129:2257–2269
    [Google Scholar]
  17. Kieser T, Bibb M. J, Buttner M. J, Chater K. F., Hopwood D. A. 2000 Practical Streptomyces Genetics Norwich: John Innes Foundation;
    [Google Scholar]
  18. Kim H. J, Calcutt M. J, Schmidt F. J., Chater K. F. 2000; Partitioning of the linear chromosome during sporulation of Streptomyces coelicolor A3(2) involves an oriC -linked parAB locus. J Bacteriol 182:1313–1320
    [Google Scholar]
  19. Larson J. L., Hershberger C. L. 1984; Shuttle vectors for cloning recombinant DNA in Escherichia coli and Streptomyces griseofuscus C581. J Bacteriol 157:314–317
    [Google Scholar]
  20. Larson J. L., Hershberger C. L. 1986; The minimal replicon of a streptomycete plasmid produces an ultrahigh level of plasmid DNA. Plasmid 15:199–209
    [Google Scholar]
  21. Lydiate D. J, Malpartida F., Hopwood D. A. 1985; The Streptomyces plasmid SCP2*: its functional analysis and development into useful cloning vectors. Gene 35:223–235
    [Google Scholar]
  22. Mahillon J., Chandler M. 1998; Insertion sequences. Microbiol Mol Biol Rev 62:725–774
    [Google Scholar]
  23. Malpartida F., Hopwood D. A. 1984; Molecular cloning of the whole biosynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host. Nature 309:462–464
    [Google Scholar]
  24. Marsh J. L, Erfle M., Wykes E. J. 1984; The pIC plasmid and phage vectors with versatile cloning sites for recombinant selection by insertional inactivation. Gene 32:481–485
    [Google Scholar]
  25. Nordström K. 1989; Mechanisms that contribute to the stable segregation of plasmids. Annu Rev Genet 23:37–69
    [Google Scholar]
  26. Pettis G. S., Cohen S. N. 1994; Transfer of the pIJ101 plasmid in Streptomyces lividans requires a cis -acting function dispensable for chromosomal gene transfer. Mol Microbiol 13:955–964
    [Google Scholar]
  27. Pridham T. G, Anderson P, Foly C, Linderfelser C. W, Hesseltine C. W., Benedict R. C. 1957; A selection of media for maintenance and taxonomic studies of Streptomyces . Antibiot Annu 1956/1957:947–953
    [Google Scholar]
  28. Rutherford K, Parkhill J, Crook J, Horsnell T, Rice P, Rajandream M. A., Barrell B. 2000; artemis: sequence visualisation and annotation. Bioinformatics 16:944–945
    [Google Scholar]
  29. Sambrook J, Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  30. Schrempf H, Bujard H, Hopwood D. A., Goebel W. 1975; Isolation of covalently closed circular desoxyribonucleic acid from Streptomyces coelicolor . J Bacteriol 121:416–421
    [Google Scholar]
  31. Servin-Gonzales L, Sampieri A, Cabello J, Galvan L, Juarez V., Castro C. 1995; Sequence and functional analysis of the Streptomyces phaeochromogenes plasmid pJV1 reveals a modular organization of Streptomyces plasmids that replicate by rolling circle. Microbiology 141:2499–2510
    [Google Scholar]
  32. Thompson C. J, Ward J. M., Hopwood D. A. 1980; DNA cloning in Streptomyces : resistance genes from antibiotic producing species. Nature 286:525–527
    [Google Scholar]
  33. Volff J.-N, Eichenser C, Viell P, Piendl W., Altenbuchner J. 1996; Nucleotide sequence and role in DNA amplification of the direct repeats composing the amplifiable element AUD1 of Streptomyces lividans 66. Mol Microbiol 21:1037–1047
    [Google Scholar]
  34. Yanisch-Perron C, Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–109
    [Google Scholar]
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