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Volume 149, Issue 8

Alteration of DNA adenine methylase (Dam) activity in causes increased spontaneous mutation frequency and attenuation in mice Free

Abstract

is one of the primary bacterial pathogens associated with bovine respiratory disease (BRD) complex. Relatively few virulence factors of have been characterized, and there is a need for improved vaccines for prevention of BRD. In other Gram-negative species, DNA adenine methylase (Dam) regulates the expression of virulence genes, and appropriate expression of Dam is required for virulence. In this study, the authors cloned and sequenced the A1 gene and demonstrated that it is able to restore Dam function in an mutant. When was placed under the control of a constitutively expressed promoter on a plasmid, it caused an increased spontaneous mutation rate in . In addition, the plasmid-mediated alteration of Dam production in caused it to be highly attenuated in mice. These findings indicate that appropriate expression of Dam is required for virulence of , which is believed to be the first report that Dam is required for virulence of a species in the . Therefore, Dam may function as a virulence gene regulator in the , similar to previously reported findings from other Gram-negative species.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): BRD, bovine respiratory disease and Dam, DNA adenine methylase
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2003-08-01
2024-04-19
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Alteration of DNA adenine methylase (Dam) activity in Pasteurella multocida causes increased spontaneous mutation frequency and attenuation in mice
Microbiology 149, 2283 (2003); https://doi.org/10.1099/mic.0.26251-0
/content/journal/micro/10.1099/mic.0.26251-0
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