RT Journal Article SR Electronic(1) A1 Alakomi, H.-L. A1 Saarela, M. A1 Helander, I. M.YR 2003 T1 Effect of EDTA on Salmonella enterica serovar Typhimurium involves a component not assignable to lipopolysaccharide release JF Microbiology, VO 149 IS 8 SP 2015 OP 2021 DO https://doi.org/10.1099/mic.0.26312-0 PB Microbiology Society, SN 1465-2080, AB The effect of EDTA on Salmonella enterica serovar Typhimurium was studied in different growth phases with cells grown with or without Ca2+ and Mg2+ supplementation. EDTA affected the outer membrane much more strongly in the early exponential phase than in the mid- or late exponential phase, as indicated by uptake of 1-N-phenylnaphthylamine (a nonpolar hydrophobic probe, M r 219), and detergent (SDS) susceptibility. This effect was, however, not paralleled by LPS release (determined by measuring LPS-specific fatty acids or 14C-labelled LPS in cell-free supernatants, per a standardized cell density), which remained unchanged as a function of the growth curve. The conclusion from these results is that in the early exponential phase the effect of EDTA in S. enterica involves a component that is independent of LPS release., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26312-0