1887

Abstract

A new type of mating, differing from classic conjugation and previously observed in a certain strain of K-12, has also been found in strains derived from ordinary F cells of K-12 exposed to an exogenous factor originating in an clinical isolate. Immunofluorescence and electron microscopy after single and double labelling of DNA were used to produce evidence in favour of a novel mating mechanism by cell contact at the poles of the bacterial rod. These findings are supported by genetic analyses indicating complete genetic mixing. Unstable complementing diploids were formed, which throw off phenotypically haploid cells, of which some showed a parental phenotype and some were true genetic recombinants. Recombination was observed even when one parent was a UV-inactivated F RecA strain. The name ‘spontaneous zygogenesis' (Z-mating, for short) is proposed for this kind of mating.

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2003-09-01
2024-04-19
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References

  1. Achtman M., Morelli G., Schwuchow S. 1978; Cell–cell interactions in conjugating Escherichia coli : role of F-pili and fate of mating aggregates. J Bacteriol 135:1053–1061
    [Google Scholar]
  2. Bachmann B. J. others 1987; Linkage map of Escherichia coli K12. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology , 7th edn. pp 1190–1219 Edited by Neidhardt F. C. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  3. Clark A. J. 1973; Recombination deficient mutants of E. coli and other bacteria. Annu Rev Genet 7:67–86
    [Google Scholar]
  4. Coetzee J. N., Sirgel F. A., Lecastsas G. 1979; Genetic recombination in fused sphaeroplasts of Providencia alcalifaciens . J Gen Microbiol 114:313–322
    [Google Scholar]
  5. Cupples C. G., Cabrera M., Cruz C., Miller J. H. 1990; A set of lacZ mutations in Escherichia coli that allow rapid detection of specific frameshift mutations. Genetics 125:275–280
    [Google Scholar]
  6. Finnegan D. F. 1976; Bacterial conjugation. In Patterns of Progress Edited by Cook J. G. Durham: Meadowfield Press;
    [Google Scholar]
  7. Fodor K., Alfoldi L. 1976; Fusion of protoplasts of Bacillus megatherium . Proc Natl Acad Sci U S A 73:2147–2150
    [Google Scholar]
  8. Grandjean V., Le Hegarat F., Hirschbein L. 1996; Prokaryotic model of epigenetic inactivation: chromosomal silencing in Bacillus subtilis fusion products. In Epigenetic Mechanisms of Gene Regulation pp 361–376 Edited by Russo V., Martiensen R. A., Riggs A. D. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  9. Gratia J. P. 1994; Ufr/s variation in Escherichia coli K12: a reversible double-mutation or alternate chromosome expression in non-complementing diploids?. Res Microbiol 145:309–325
    [Google Scholar]
  10. Gratia J. P., Deschuyteneer R. 1998; A sensitive test for detecting and analysing products of ‘illegitimate mating’ in Enterobacteria . Arch Physiol Biochem 106:B11
    [Google Scholar]
  11. Hane M. W., Wood T. H. 1969; Escherichia coli K12 mutants resistant to nalidixic acid: genetic mapping and dominance studies. J Bacteriol 99:239–241
    [Google Scholar]
  12. Hayes W. 1968 The Genetics of Bacteria and Their Viruses , 2nd edn. Oxford: Blackwell Scientific Publications;
  13. Hopwood D. A., Wright H. M., Bibb M. J., Cohen S. N. 1977; Genetic recombination through protoplast fusion in Streptomyces . Nature 268:171–174
    [Google Scholar]
  14. Hotchkiss R. P., Gabor M. H. 1980; Biparental products of bacterial protoplasts fusion showing unequal chromosome expression. Proc Natl Acad Sci U S A 77:3553–3557
    [Google Scholar]
  15. Lederberg J. 1949; Aberrant heterozygotes in Escherichia coli . Proc Natl Acad Sci U S A 35:178–184
    [Google Scholar]
  16. Lederberg J. 1951; Streptomycin-resistance: a genetically recessive mutation. J Bacteriol 61:549–550
    [Google Scholar]
  17. Lederberg J., St Clair J. 1958; Protoplasts and L-type growth of Escherichia coli . J Bacteriol 75:143–160
    [Google Scholar]
  18. Low B. 1965; Low recombination frequency for markers very near the origin in conjugation in E. coli . Genet Res 6:469–473
    [Google Scholar]
  19. Low B. 1968; Formation of merodiploids in matings with a class of Rec recipient strains of Escherichia coli K12. Proc Natl Acad Sci U S A 60:160–167
    [Google Scholar]
  20. Miller J. H. 1972 Experiments in Molecular Genetics Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
  21. Porter R. D. 1988; Modes of gene transfer in bacteria. In Genetic Recombination pp 1–41 Edited by Kucherlapati R., Smith G. R. Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Schaeffer P., Cami B., Hotchkiss R. D. 1976; Fusion of bacterial protoplasts. Proc Natl Acad Sci U S A 73:2151–2155
    [Google Scholar]
  23. Thiry M. 1992; Highly sensitive immunodetection of DNA on sections with exogenous terminal deoxynucleotidyl transferase and non-isotopic nucleotide analogs. J Histochem Cytochem 40:411–419
    [Google Scholar]
  24. Thiry M. 1999; Ultrastructural methods for nucleic acid detection by immunocytology. Prog Histochem Cytochem 34:87–160
    [Google Scholar]
  25. Tyurin M. V., Doroshenko V. G., Oparina N. Yu. 1997; Electro-fusion of Escherichia coli cells. Membr Cell Biol 11:121–129
    [Google Scholar]
  26. Zelle M. R., Lederberg J. 1951; Single-cell isolations of diploid heterozygous Escherichia coli . J Bacteriol 61:351–355
    [Google Scholar]
  27. Zipkas D., Riley M. 1976; Simplified method for interruption of conjugation in Escherichia coli . J Bacteriol 126:559–560
    [Google Scholar]
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