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Volume 149, Issue 10

A cryptic plasmid of encodes a conjugative transfer system related to the regions of CloDF13 Mob and IncX Pil Free

Abstract

29930 (biotype 1A; O : 7,8), the producing strain of the phage-tail-like bacteriocin enterocoliticin, possesses a plasmid-encoded conjugative type IV transfer system. The genes of the conjugative system were found by screening of a cosmid library constructed from total DNA of strain 29930. The cosmid Cos100 consists of the vector SuperCos1 and an insert DNA of 40 303 bp derived from a cryptic plasmid of strain 29930. The conjugative transfer system consists of genes encoding a DNA transfer and replication system (Dtr) with close relationship to the region of the mobilizable plasmid CloDF13 and a gene cluster encoding a mating pair formation system (Mpf) closely related to the Mpf system of the IncX plasmid R6K. However, a gene encoding a homologue of TaxB, the coupling protein of the IncX system, is missing. The whole transfer region has a size of approximately 17 kb. The recombinant plasmid Cos100 was shown to be transferable between and with transfer frequencies up to 0·1 transconjugants per donor. Mutations generated by inserting a tetracycline cassette into putative genes yielded a transfer-deficient phenotype. Conjugative transfer of the cryptic plasmid could not be demonstrated in the original host 29930. However, a kanamycin-resistance-conferring derivative of the plasmid was successfully introduced into K-12 by transformation and was shown to be self-transmissible. Furthermore, Southern blot hybridization and PCR experiments were carried out to elucidate the distribution of the conjugative transfer system in . In total, six biotype 1A strains harbouring closely related systems on endogenous plasmids were identified.

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Keyword(s): T4SS, type IV secretion/transfer system
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2003-10-01
2024-04-19
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402
     [Google Scholar]
  2. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1987; Preparation and analysis of DNA. In Current Protocols in Molecular Biology pp 2.0.1–2.14.8 New York: Wiley;
     [Google Scholar]
  3. Baron C., O'Callaghan D., Lanka E. 2002; Bacterial secrets of secretion: EuroConference on the biology of type IV secretion processes. Mol Microbiol 43:1359–1365
     [Google Scholar]
  4. Bayer M., Eferl R., Zellnig G., Teferle K., Dijkstra A., Koraimann G., Högenauer G. 1995; Gene 19 of plasmid R1 is required for both efficient conjugative DNA transfer and bacteriophage R17 infection. J Bacteriol 177:4279–4288
     [Google Scholar]
  5. Belogurov A. A., Delver E. P., Agafonova O. V., Belogurova N. G., Lee L. Y., Kado C. I. 2000; Antirestriction protein Ard (type C) encoded by IncW plasmid pSa has a high similarity to the “protein transport” domain of TraC1 primase of promiscuous plasmid RP4. J Mol Biol 296:969–977
     [Google Scholar]
  6. Bercovier H., Mollaret H. H. others 1984; Yersinia . In Bergey's Manual of Systematic Bacteriology pp  498–506 Edited by Krieg N. R. Baltimore: Williams & Wilkins;
     [Google Scholar]
  7. Betteridge T. J., Yang J., Pittard A. J., Praszkier J. 2003; Interaction of the initiator protein of an IncB plasmid with its origin of DNA replication. J Bacteriol 185:2210–2218
     [Google Scholar]
  8. Bignell C., Thomas C. M. 2001; The bacterial ParA-ParB partitioning proteins. J Biotechnol 91:1–34
     [Google Scholar]
  9. Birnboim H. C., Doly J. 1979; A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res 7:1513–1523
     [Google Scholar]
  10. Bottone E. J. 1997; Yersinia enterocolitica : the charisma continues. Clin Microbiol Rev 10:257–276
     [Google Scholar]
  11. Burnens A. P., Frey A., Nicolet J. 1996; Association between clinical presentation, biogroups and virulence attributes of Yersinia enterocolitica strains in human diarrhoeal disease. Epidemiol Infect 116:27–34
     [Google Scholar]
  12. Burns D. L. 1999; Biochemistry of type IV secretion. Curr Opin Microbiol 2:25–29
     [Google Scholar]
  13. Cao T. B., Saier M. H. Jr 2001; Conjugal type IV macromolecular transfer systems of Gram-negative bacteria: organismal distribution, structural constraints and evolutionary conclusions. Microbiology 147:3201–3214
     [Google Scholar]
  14. Carniel E. 2002; Plasmids and pathogenicity islands of Yersinia . Curr Top Microbiol Immunol 264:89–108
     [Google Scholar]
  15. Chang A. C., Cohen S. N. 1978; Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid. J Bacteriol 134:1141–1156
     [Google Scholar]
  16. Christie P. J. 2001; Type IV secretion: intercellular transfer of macromolecules by systems ancestrally related to conjugation machines. Mol Microbiol 40:294–305
     [Google Scholar]
  17. Christie P. J., Vogel J. P. 2000; Bacterial type IV secretion: conjugation systems adapted to deliver effector molecules to host cells. Trends Microbiol 8:354–360
     [Google Scholar]
  18. Cornelis G. R. 2002; Yersinia type III secretion: send in the effectors. J Cell Biol 158:401–408
     [Google Scholar]
  19. Covarrubias L., Cervantes L., Covarrubias A., Soberon X., Vichido I., Blanco A., Kupersztoch-Portnoy Y. M., Bolivar F. 1981; Construction and characterization of new cloning vehicles. V. Mobilization and coding properties of pBR322 and several deletion derivatives including pBR327 and pBR328. Gene 13:25–35
     [Google Scholar]
  20. de Lorenzo V., Herrero M., Jakubzik U., Timmis K. N. 1990; Mini-Tn5 transposon derivatives for insertion mutagenesis, promoter probing, and chromosomal insertion of cloned DNA in gram-negative eubacteria. J Bacteriol 172:6568–6572
     [Google Scholar]
  21. Dong X. Q., Lindler L. E., Chu M. C. 2000; Complete DNA sequence and analysis of an emerging cryptic plasmid isolated from Yersinia pestis . Plasmid 43:144–148
     [Google Scholar]
  22. Galli D. M., Chen J., Novak K. F., Leblanc D. J. 2001; Nucleotide sequence and analysis of conjugative plasmid pVT745. J Bacteriol 183:1585–1594
     [Google Scholar]
  23. Goelz G., Knabner D., Appel B., Strauch E. 2003; A conjugal type IV transfer system in Yersinia enterocolitica strains. Adv Exp Med Biol 529:51–53
     [Google Scholar]
  24. Gomis-Rüth F. X., Moncalian G., de la Cruz F., Coll M. 2002; Conjugative plasmid protein TrwB, an integral membrane type IV secretion system coupling protein. Detailed structural features and mapping of the active site cleft. J Biol Chem 277:7556–7566
     [Google Scholar]
  25. Grant T., Bennett-Wood V., Robins-Browne R. M. 1999; Characterization of the interaction between Yersinia enterocolitica biotype 1A and phagocytes and epithelial cells in vitro . Infect Immun 67:4367–4375
     [Google Scholar]
  26. Hapfelmeier S., Domke N., Zambryski P. C., Baron C. 2000; VirB6 is required for stabilization of VirB5 and VirB3 and formation of VirB7 homodimers in Agrobacterium tumefaciens . J Bacteriol 182:4505–4511
     [Google Scholar]
  27. Hayes F. 2000; The partition system of multidrug resistance plasmid TP228 includes a novel protein that epitomizes an evolutionarily distinct subgroup of the ParA superfamily. Mol Microbiol 37:528–541
     [Google Scholar]
  28. Herrero M., de Lorenzo V., Timmis K. N. 1990; Transposon vectors containing non-antibiotic resistance selection markers for cloning and stable chromosomal insertion of foreign genes in gram-negative bacteria. J Bacteriol 172:6557–6567
     [Google Scholar]
  29. Hoffmann B., Strauch E., Gewinner C., Nattermann H., Appel B. 1998; Characterization of plasmid regions of foodborne Yersinia enterocolitica biogroup 1A strains hybridizing to the Yersinia enterocolitica virulence plasmid. Syst Appl Microbiol 21:201–211
     [Google Scholar]
  30. Hueck C. J. 1998; Type III protein secretion systems in bacterial pathogens of animals and plants. Microbiol Mol Biol Rev 62:379–433
     [Google Scholar]
  31. Kado C. I., Liu S. T. 1981; Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol 145:1365–1373
     [Google Scholar]
  32. Kalkum M., Eisenbrandt R., Lurz R., Lanka E. 2002; Tying rings for sex. Trends Microbiol 10:382–387
     [Google Scholar]
  33. Krause S., Bárcena M., Pansegrau W., Lurz R., Carazo J. M., Lanka E. 2000; Sequence-related protein export NTPases encoded by the conjugative transfer region of RP4 and by the cag pathogenicity island of Helicobacter pylori share similar hexameric ring structures. Proc Natl Acad Sci U S A 97:3067–3072
     [Google Scholar]
  34. Lai E. M., Kado C. I. 2000; The T-pilus of Agrobacterium tumefaciens . Trends Microbiol 8:361–369
     [Google Scholar]
  35. Lewin A., Strauch E., Hertwig S., Hoffmann B., Nattermann H., Appel B. 1996; Comparison of plasmids of strains of Yersinia enterocolitica biovar 1A with the virulence plasmid of a pathogenic Y. enterocolitica strain. Zentbl Bakteriol 285:52–63
     [Google Scholar]
  36. Li Z., Hiasa H., Kumar U., DiGate R. J. 1997; The traE gene of plasmid RP4 encodes a homologue of Escherichia coli DNA topoisomerase III. J Biol Chem 272:19582–19587
     [Google Scholar]
  37. Llosa M., Gomis-Rüth F. X., Coll M., de la Cruz F. 2002; Bacterial conjugation: a two-step mechanism for DNA transport. Mol Microbiol 45:1–8
     [Google Scholar]
  38. McGhee G. C., Jones A. L. 2000; Complete nucleotide sequence of ubiquitous plasmid pEA29 from Erwinia amylovora strain Ea88: gene organization and intraspecies variation. Appl Environ Microbiol 66:4897–4907
     [Google Scholar]
  39. Núñez B., de la Cruz F. 2001; Two atypical mobilization proteins are involved in plasmid CloDF13 relaxation. Mol Microbiol 39:1088–1099
     [Google Scholar]
  40. Núñez B., Avila P., de la Cruz F. 1997; Genes involved in conjugative DNA processing of plasmid R6K. Mol Microbiol 24:1157–1168
     [Google Scholar]
  41. Paetzel M., Dalbey R. E., Strynadka N. C. 2000; The structure and mechanism of bacterial type I signal peptidases. A novel antibiotic target. Pharmacol Ther 87:27–49
     [Google Scholar]
  42. Page A. L., Parsot C. 2002; Chaperones of the type III secretion pathway: jacks of all trades. Mol Microbiol 46:1–11
     [Google Scholar]
  43. Pansegrau W., Lanka E. 1996; Enzymology of DNA transfer by conjugative mechanisms. Prog Nucleic Acid Res Mol Biol 54:197–251
     [Google Scholar]
  44. Pohlman R. F., Liu F., Wang L., More M. I., Winans S. C. 1993; Genetic and biochemical analysis of an endonuclease encoded by the IncN plasmid pKM101. Nucleic Acids Res 21:4867–4872
     [Google Scholar]
  45. Praszkier J., Wei T., Siemering K., Pittard J. 1991; Comparative analysis of the replication regions of IncB, IncK, and IncZ plasmids. J Bacteriol 173:2393–2397
     [Google Scholar]
  46. Rabel C., Grahn A. M., Lurz R., Lanka E. 2003; The VirB4 family of proposed traffic nucleoside triphosphatases: common motifs in plasmid RP4 TrbE are essential for conjugation and phage adsorption. J Bacteriol 185:1045–1058
     [Google Scholar]
  47. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual , 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
     [Google Scholar]
  48. Schneiker S., Keller M., Dröge M., Lanka E., Pühler A., Selbitschka W. 2001; The genetic organization and evolution of the broad host range mercury resistance plasmid pSB102 isolated from a microbial population residing in the rhizosphere of alfalfa. Nucleic Acids Res 29:5169–5181
     [Google Scholar]
  49. Schrammeijer B., den Dulk-Ras A., Vergunst A. C., Jurado J. E., Hooykaas P. J. J. 2003; Analysis of Vir protein translocation from Agrobacterium tumefaciens using Saccharomyces cerevisiae as a model: evidence for transport of a novel effector protein VirE3. Nucleic Acids Res 31:860–868
     [Google Scholar]
  50. Sherrat D. 1989; Tn 3 and related transposable elements: site-specific recombination and transposition. In Mobile DNA pp  163–184 Edited by Berg D. E., Howe M. M. Washington, DC: American Society for Microbiology;
     [Google Scholar]
  51. Simon R., Priefer U., Pühler A. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram-negative bacteria. Biotechnology 1:784–791
     [Google Scholar]
  52. Snellings N. J., Popek M., Lindler L. E. 2001; Complete DNA sequence of Yersinia enterocolitica serotype O : 8 low-calcium-response plasmid reveals a new virulence plasmid-associated replicon. Infect Immun 69:4627–4638
     [Google Scholar]
  53. Strauch E., Voigt I., Broll H., Appel B. 2000a; Use of a plasmid of a Yersinia enterocolitica biogroup 1A strain for the construction of cloning vectors. J Biotechnol 79:63–72
     [Google Scholar]
  54. Strauch E., Hoffmann B., Heins G., Appel B. 2000b; Isolation of a new insertion element of Yersinia intermedia closely related to remnants of mobile genetic elements present on Yersinia plasmids harboring the Yop virulon. FEMS Microbiol Lett 193:37–44
     [Google Scholar]
  55. Strauch E., Kaspar H., Schaudinn C., Dersch P., Madela K., Gewinner C., Hertwig S., Wecke J., Appel B. 2001; Characterization of enterocoliticin, a phage tail-like bacteriocin, and its effect on pathogenic Yersinia enterocolitica strains. Appl Environ Microbiol 67:5634–5642
     [Google Scholar]
  56. Willetts N., Wilkins B. 1984; Processing of plasmid DNA during conjugation. Microbiol Rev 48:24–41
     [Google Scholar]
  57. Wu C. Y., Fu J. F., Liu S. T. 2001; The replicon of pSW800 from Pantoea stewartii . Microbiology 147:2757–2767
     [Google Scholar]
  58. Yarmolinsky M. 2000; Transcriptional silencing in bacteria. Curr Opin Microbiol 3:138–143
     [Google Scholar]
  59. Yeo H. J., Savvides S. N., Herr A. B., Lanka E., Waksman G. 2000; Crystal structure of the hexameric traffic ATPase of the Helicobacter pylori type IV secretion system. Mol Cell 6:1461–1472
     [Google Scholar]
  60. Young G. M., Schmiel D. H., Miller V. L. 1999; A new pathway for the secretion of virulence factors by bacteria: the flagellar export apparatus functions as a protein-secretion system. Proc Natl Acad Sci U S A 96:6456–6461
     [Google Scholar]
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A cryptic plasmid of Yersinia enterocolitica encodes a conjugative transfer system related to the regions of CloDF13 Mob and IncX Pil
Microbiology 149, 2829 (2003); https://doi.org/10.1099/mic.0.26418-0
/content/journal/micro/10.1099/mic.0.26418-0
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