RT Journal Article SR Electronic(1) A1 Arous, Safia A1 Buchrieser, Carmen A1 Folio, Patrice A1 Glaser, Philippe A1 Namane, Abdelkader A1 Hébraud, Michel A1 Héchard, YannYR 2004 T1 Global analysis of gene expression in an rpoN mutant of Listeria monocytogenes JF Microbiology, VO 150 IS 5 SP 1581 OP 1590 DO https://doi.org/10.1099/mic.0.26860-0 PB Microbiology Society, SN 1465-2080, AB The role of the alternative σ 54 factor, encoded by the rpoN gene, was investigated in Listeria monocytogenes by comparing the global gene expression of the wild-type EGDe strain and an rpoN mutant. Gene expression, using whole-genome macroarrays, and protein content, using two-dimensional gel electrophoresis, were analysed. Seventy-seven genes and nine proteins, whose expression was modulated in the rpoN mutant as compared to the wild-type strain, were identified. Most of the modifications were related to carbohydrate metabolism and in particular to pyruvate metabolism. However, under the conditions studied, only the mptACD operon was shown to be directly controlled by σ 54. Therefore, the remaining modifications seem to be due to indirect effects. In parallel, an in silico analysis suggests that σ 54 may directly control the expression of four different phosphotransferase system (PTS) operons, including mptACD. PTS activity is known to have a direct effect on the pyruvate pool and on catabolite regulation. These results suggest that σ 54 is mainly involved in the control of carbohydrate metabolism in L. monocytogenes via direct regulation of PTS activity, alteration of the pyruvate pool and modulation of carbon catabolite regulation., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/mic.0.26860-0