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Volume 150, Issue 7

Extended phenotype of an -like mutant in Free

Abstract

Tn mutagenesis was used to generate an SPF94 mutant. Genetic analysis of this mutant revealed that a homologue of the gene, which controls cell shape in and , was inactivated. The cell-surface properties of the mutant were different from those of the parental strain. The mutant colonies were highly fluorescent when grown on plates containing Calcofluor White. Light and electron microscopy revealed that the mutant cells were round and had thicker capsules than the spiral parental strain. The mutants contained up to ten times more capsule protein than the parental strain, but lacked a 40 kDa protein that is abundant in the parental strain. The phenotype of the isolated mutant resembled that of the cyst-like differentiated forms of , suggesting that the homologue could be involved in differentiation.

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Keyword(s): CFW, Calcofluor White , PS, polysaccharide and SEM, scanning electron microscopy
SGM
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2004-07-01
2024-04-25
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Extended phenotype of an mreB-like mutant in Azospirillum brasilense
Microbiology 150, 2465 (2004); https://doi.org/10.1099/mic.0.26904-0
/content/journal/micro/10.1099/mic.0.26904-0
/content/journal/micro/10.1099/mic.0.26904-0
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