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Volume 150, Issue 12

New endophytic isolates of , a volatile-antibiotic-producing fungus Free

Abstract

, an endophytic fungus originally isolated from , produces a mixture of volatile organic compounds (VOCs) in culture and its spectrum of antimicrobial activity is broad. Using the original isolate of as a selection tool, it has been possible to find other culturally and biochemically unique wild-type isolates of this organism existing as endophytes in a variety of other plant species, including (fern-leafed grevillea), (snake vine) and (nanka bakarra) growing in the northern reaches of the Northern Territory of Australia. Interestingly, none of the new isolates had a culture morphology that was identical to the original isolate, nevertheless each possessed hyphal characteristics that resembled that isolate. Furthermore, their ITS-5·8S rDNA sequences were 96–99 % identical to that of and the isolates were considered on the basis of the DNA sequence data. However, the VOCs produced by these new isolates greatly differed in quality from the original strain by virtue of the production of naphthalene, naphthalene, 1,1′-oxybis-, and one or more other compounds. In bioassays with a range of test micro-organisms, including fungi and bacteria, each isolate possessed biological activity but the range of activity was great. Artificial mixtures of some of the VOCs mimicked the effects of the VOCs of the fungus. The value of these observations to the biology and practical uses of in agriculture and other applications is discussed.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): ITS, internal transcribed spacer and VOC, volatile organic compound
© SGM
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2004-12-01
2024-04-20
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References

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New endophytic isolates of Muscodor albus, a volatile-antibiotic-producing fungus
Microbiology 150, 4023 (2004); https://doi.org/10.1099/mic.0.27334-0
/content/journal/micro/10.1099/mic.0.27334-0
/content/journal/micro/10.1099/mic.0.27334-0
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vol. , part 12, pp. 4023 - 4031

Nucleotide sequence comparisons in the ITS-5.8S rDNA between the different isolates of are shown in the accompanying PDF.

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